TY - JOUR
T1 - A comparison of salivary testosterone measurement using immunoassays and tandem mass spectrometry
AU - Welker, Keith M.
AU - Lassetter, Bethany
AU - Brandes, Cassandra M.
AU - Prasad, Smrithi
AU - Koop, Dennis R.
AU - Mehta, Pranjal H.
N1 - Funding Information:
This research was supported by two National Science Foundation grants awarded to PHM (Award Numbers: 1451848 and 1303743 ). This funding sponsor was not involved in study design; in the collection, analysis and interpretation of data; in the writing of the report; or in the decision to submit the article for publication.
Publisher Copyright:
© 2016 Elsevier Ltd.
PY - 2016/9/1
Y1 - 2016/9/1
N2 - Enzyme immunoassays (EIAs) are widely used to measure salivary testosterone. However, little is known about how accurately different EIAs assess testosterone, partially because estimates across various EIAs differ considerably. We compared testosterone concentrations across EIAs of three commonly used manufacturers (DRG International, Salimetrics, and IBL International) to liquid chromatography tandem mass spectrometry (LC-MS/MS). Relative to EIAs from Salimetrics and IBL International, EIAs supplied by DRG International provided the closest approximation to LC-MS/MS testosterone concentrations, followed closely by EIAs from Salimetrics, and then IBL. Additionally, EIAs tended to inflate estimates of lower testosterone concentrations in women. Examining our results and comparing them to existing data revealed that testosterone EIAs had decreased linear correspondence with LC-MS/MS in comparison to cortisol EIAs. Overall, this paper provides researchers with information to better measure testosterone in their research and more accurately compare testosterone measurements across different methods.
AB - Enzyme immunoassays (EIAs) are widely used to measure salivary testosterone. However, little is known about how accurately different EIAs assess testosterone, partially because estimates across various EIAs differ considerably. We compared testosterone concentrations across EIAs of three commonly used manufacturers (DRG International, Salimetrics, and IBL International) to liquid chromatography tandem mass spectrometry (LC-MS/MS). Relative to EIAs from Salimetrics and IBL International, EIAs supplied by DRG International provided the closest approximation to LC-MS/MS testosterone concentrations, followed closely by EIAs from Salimetrics, and then IBL. Additionally, EIAs tended to inflate estimates of lower testosterone concentrations in women. Examining our results and comparing them to existing data revealed that testosterone EIAs had decreased linear correspondence with LC-MS/MS in comparison to cortisol EIAs. Overall, this paper provides researchers with information to better measure testosterone in their research and more accurately compare testosterone measurements across different methods.
KW - Immunoassays
KW - Liquid chromatography
KW - Mass spectrometry
KW - Measurement
KW - Salivary testosterone
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U2 - 10.1016/j.psyneuen.2016.05.022
DO - 10.1016/j.psyneuen.2016.05.022
M3 - Article
C2 - 27295182
AN - SCOPUS:84973484166
SN - 0306-4530
VL - 71
SP - 180
EP - 188
JO - Psychoneuroendocrinology
JF - Psychoneuroendocrinology
ER -