Abstract
Background: Constitutively active mutations (CAMs) affect the dynamics of G protein-coupled receptors (GPCRs), through undefined mechanisms. Results: Site-directed fluorescence labeling (SDFL) studies find a CAM (M257Y) alters the dynamics of the GPCR ligand-free opsin. Conclusion: The M257Y CAM alters the dynamics of the conversion between active and inactive opsin conformations. Significance: SDFL can be used to gain insights into how CAMs affect the dynamics of G protein-coupled receptors.
Original language | English (US) |
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Pages (from-to) | 28207-28216 |
Number of pages | 10 |
Journal | Journal of Biological Chemistry |
Volume | 288 |
Issue number | 39 |
DOIs | |
State | Published - Sep 27 2013 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology