@article{5cb388b397bd4a9ebe084d9f0c8eb633,
title = "A Diselenide Turn-On Fluorescent Probe for the Detection of Thioredoxin Reductase",
abstract = "We report the first diselenide-based probe for the selective detection of thioredoxin reductase (TrxR), an enzyme commonly overexpressed in melanomas. The probe design involves conjugation of a seminaphthorhodafluor dye with a diselenide moiety. TrxR reduces the diselenide bond, triggering a fluorescence turn-on response of the probe. Kinetic studies reveal favorable binding of the probe with TrxR with a Michaelis–Menten constant (Km) of 15.89 μm. Computational docking simulations predict a greater binding affinity to the TrxR active site in comparison to its disulfide analogue. In vitro imaging studies further confirmed the diselenide probe exhibited improved signaling of TrxR activity compared to the disulfide analogue.",
keywords = "diagnostics, melanoma, selenium probes, seminaphthorhodafluor, thioredoxin reductase",
author = "Mafireyi, {Tendai J.} and Madeleine Laws and Bassett, {John W.} and Cassidy, {Pamela B.} and Escobedo, {Jorge O.} and Strongin, {Robert M.}",
note = "Funding Information: This work was supported by the National Institutes of Health (grant R15EB016870). Molecular graphics and analyses performed with UCSF Chimera, developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, with support from NIH P41‐GM103311. Funding Information: This work was supported by the National Institutes of Health (grant R15EB016870). Molecular graphics and analyses performed with UCSF Chimera, developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, with support from NIH P41-GM103311. Publisher Copyright: {\textcopyright} 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2020",
month = aug,
day = "24",
doi = "10.1002/anie.202004094",
language = "English (US)",
volume = "59",
pages = "15147--15151",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "35",
}