TY - JOUR
T1 - A family of acid-sensing ion channels from the zebrafish
T2 - Widespread expression in the central nervous system suggests a conserved role in neuronal communication
AU - Paukert, Martin
AU - Sidi, Samuel
AU - Russell, Claire
AU - Siba, Maria
AU - Wilson, Stephen W.
AU - Nicolson, Teresa
AU - Gründer, Stefan
PY - 2004/4/30
Y1 - 2004/4/30
N2 - Acid-sensing ion channels (ASICs) are excitatory receptors for extracellular H+. Proposed functions include synaptic transmission, peripheral perception of pain, and mechanosensation. Despite the physiological importance of these functions, the precise role of ASICs has not yet been established. In order to increase our understanding of the physiological role and basic structure-function relationships of ASICs, we report here the cloning of six new ASICs from the zebrafish (zASICs). zASICs possess the basic functional properties of mammalian ASICs: activation by extracellular H +, Na+ selectivity, and block by micromolar concentrations of amiloride. The zasic genes are broadly expressed in the central nervous system, whereas expression in the peripheral nervous system is scarce. This pattern suggests a predominant role for zASICs in neuronal communication. Our results suggest a conserved function for receptors of extracellular H+ in the central nervous system of vertebrates.
AB - Acid-sensing ion channels (ASICs) are excitatory receptors for extracellular H+. Proposed functions include synaptic transmission, peripheral perception of pain, and mechanosensation. Despite the physiological importance of these functions, the precise role of ASICs has not yet been established. In order to increase our understanding of the physiological role and basic structure-function relationships of ASICs, we report here the cloning of six new ASICs from the zebrafish (zASICs). zASICs possess the basic functional properties of mammalian ASICs: activation by extracellular H +, Na+ selectivity, and block by micromolar concentrations of amiloride. The zasic genes are broadly expressed in the central nervous system, whereas expression in the peripheral nervous system is scarce. This pattern suggests a predominant role for zASICs in neuronal communication. Our results suggest a conserved function for receptors of extracellular H+ in the central nervous system of vertebrates.
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U2 - 10.1074/jbc.M401477200
DO - 10.1074/jbc.M401477200
M3 - Article
C2 - 14970195
AN - SCOPUS:2442550900
SN - 0021-9258
VL - 279
SP - 18783
EP - 18791
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 18
ER -