Abstract
We present a practical method for the rescue of previosly stable hybridoma clones which increases the proportion of desired cells in the population before cloning by limiting dilution. When the antibody activity of a culture supernatant was lower than that previously obtained, a precloning distribution at a density of 10 cells per microtiter well greatly improved the chances of obtaining a single active clone by subsequent limiting dilution. The Poisson distribution model was used to evaluate the method. Probabilities calculated clearly demonstrate the advantage of this precloning distribution step when attempting to isolate a hydridoma cell line that is relatively rare in a population.
Original language | English (US) |
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Pages (from-to) | 1141-1146 |
Number of pages | 6 |
Journal | In Vitro Cellular & Developmental Biology |
Volume | 25 |
Issue number | 12 |
DOIs | |
State | Published - Dec 1989 |
Externally published | Yes |
Keywords
- hybridoma
- limiting dilution cloning
- monoclonal antibody
ASJC Scopus subject areas
- Developmental Biology
- Clinical Biochemistry
- Cell Biology