TY - JOUR
T1 - A procedure to produce high alcohol intake in mice
AU - Finn, Deborah A.
AU - Belknap, John K.
AU - Cronise, Kim
AU - Yoneyama, Naomi
AU - Murillo, Andrea
AU - Crabbe, John C.
N1 - Funding Information:
Acknowledgements This study was supported by NIAAA INIA Consortium grants AA13478 and AA13519, Portland Alcohol Research Center grant AA10760, and the Department of Veterans Affairs. We gratefully acknowledge the expert technical assistance of Stacy Matthews, Season Long and Michelle Tanchuck in some of the earlier studies.
PY - 2005/4
Y1 - 2005/4
N2 - Rationale: While prolonged access to ethanol (EtOH), or deprivations, or their combination have occasionally been shown to yield high levels of voluntary self-administration, in almost all cases, rodents do not self-administer alcohol to the degree that they will develop substantial, intoxicating blood alcohol levels and then continue to self-administer at these levels. Objectives: The purpose of the present series of experiments was to modify a fluid restriction procedure to demonstrate consistent, high EtOH consumption. Methods: Male and female mice from an alcohol preferring inbred strain (C57BL/6J; B6) as well as from a genetically heterogeneous strain (WSC) were given varying periods of access to fluid, ranging from 90 min to 10 h per day, for 12-21 days. Every 3rd or 4th day, separate groups of mice were offered a 5, 7 or 10% EtOH solution for either 10 min or 30 min, followed by water for the remainder of the time. Results: In all studies, stable high EtOH doses were consumed by both B6 and WSC mice across the EtOH sessions, exceeding 2 g/kg in a 30-min session. Mean blood EtOH concentration exceeded 1 mg/ml (i.e. 100 mg%), with values in individual animals ranging from 0.6 mg/ml to 3.4 mg/ml. Notably, mice receiving 10 h of fluid/day continued to consume 2 g/kg doses of EtOH. While this procedure did not produce subsequent preference for EtOH in WSC mice, consumption remained high in some animals. Conclusions: These data indicate that scheduling fluid intake produces high, stable EtOH consumption and BEC in male and female B6 and WSC mice.
AB - Rationale: While prolonged access to ethanol (EtOH), or deprivations, or their combination have occasionally been shown to yield high levels of voluntary self-administration, in almost all cases, rodents do not self-administer alcohol to the degree that they will develop substantial, intoxicating blood alcohol levels and then continue to self-administer at these levels. Objectives: The purpose of the present series of experiments was to modify a fluid restriction procedure to demonstrate consistent, high EtOH consumption. Methods: Male and female mice from an alcohol preferring inbred strain (C57BL/6J; B6) as well as from a genetically heterogeneous strain (WSC) were given varying periods of access to fluid, ranging from 90 min to 10 h per day, for 12-21 days. Every 3rd or 4th day, separate groups of mice were offered a 5, 7 or 10% EtOH solution for either 10 min or 30 min, followed by water for the remainder of the time. Results: In all studies, stable high EtOH doses were consumed by both B6 and WSC mice across the EtOH sessions, exceeding 2 g/kg in a 30-min session. Mean blood EtOH concentration exceeded 1 mg/ml (i.e. 100 mg%), with values in individual animals ranging from 0.6 mg/ml to 3.4 mg/ml. Notably, mice receiving 10 h of fluid/day continued to consume 2 g/kg doses of EtOH. While this procedure did not produce subsequent preference for EtOH in WSC mice, consumption remained high in some animals. Conclusions: These data indicate that scheduling fluid intake produces high, stable EtOH consumption and BEC in male and female B6 and WSC mice.
KW - Blood ethanol concentration
KW - C57BL/6
KW - Ethanol consumption
KW - Genetically heterogeneous mice
KW - Intoxication
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U2 - 10.1007/s00213-004-2039-8
DO - 10.1007/s00213-004-2039-8
M3 - Article
C2 - 15765261
AN - SCOPUS:16844377776
SN - 0033-3158
VL - 178
SP - 471
EP - 480
JO - Psychopharmacology
JF - Psychopharmacology
IS - 4
ER -