A rapid, nongenomic action of glucocorticoids in rat B103 neuroblastoma cells

Jian Zhong Han; Wen Lin; Shu Jie Lou; Jian Qiu; Yi Zhang Chen

doi:10.1016/S0167-4889(02)00242-2

A rapid, nongenomic action of glucocorticoids in rat B103 neuroblastoma cells

Jian Zhong Han, Wen Lin, Shu Jie Lou, Jian Qiu, Yi Zhang Chen

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

We report here a new example in which glucocorticoids (GCs) acted in a rapid, nongenomic way. In rat B103 neuroblastoma cells, 5-hydroxytryptamine (5-HT) was found to evoke an immediate rise in intracellular free calcium concentration ([Ca²⁺]_i). Pre-incubation of B103 cells for 5 min with corticosterone (B) or bovine serum albumin-conjugated corticosterone (B-BSA) concentration-dependently (10^-4-10^-8 M) inhibited the peak increments in [Ca²⁺]_i. Cortisol and dexamethasone had a similar effect, while deoxycorticosterone and cholesterol were ineffective. This rapid inhibitory effect of corticosterone could be mimicked by protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) and abolished completely by PKC inhibitors Ro31-8220 or GF-109203X. Neither pertussis toxin (PTX) nor nuclear GC receptor (GR) antagonist RU38486 influenced the rapid action of B. Our results suggest that GCs can modulate the 5-HT-induced Ca²⁺ response in B103 cells in a membrane-initiated, nongenomic, and PKC-dependent manner.

Original language	English (US)
Pages (from-to)	21-27
Number of pages	7
Journal	Biochimica et Biophysica Acta - Molecular Cell Research
Volume	1591
Issue number	1-3
DOIs	https://doi.org/10.1016/S0167-4889(02)00242-2
State	Published - Aug 19 2002
Externally published	Yes

Keywords

5-Hydroxytryptamine
Calcium
Glucocorticoid
Protein kinase C

ASJC Scopus subject areas

Molecular Biology
Cell Biology

Access to Document

10.1016/S0167-4889(02)00242-2

Cite this

@article{f887eca6daf64cae87f0770ddd89777e,

title = "A rapid, nongenomic action of glucocorticoids in rat B103 neuroblastoma cells",

abstract = "We report here a new example in which glucocorticoids (GCs) acted in a rapid, nongenomic way. In rat B103 neuroblastoma cells, 5-hydroxytryptamine (5-HT) was found to evoke an immediate rise in intracellular free calcium concentration ([Ca2+]i). Pre-incubation of B103 cells for 5 min with corticosterone (B) or bovine serum albumin-conjugated corticosterone (B-BSA) concentration-dependently (10-4-10-8 M) inhibited the peak increments in [Ca2+]i. Cortisol and dexamethasone had a similar effect, while deoxycorticosterone and cholesterol were ineffective. This rapid inhibitory effect of corticosterone could be mimicked by protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) and abolished completely by PKC inhibitors Ro31-8220 or GF-109203X. Neither pertussis toxin (PTX) nor nuclear GC receptor (GR) antagonist RU38486 influenced the rapid action of B. Our results suggest that GCs can modulate the 5-HT-induced Ca2+ response in B103 cells in a membrane-initiated, nongenomic, and PKC-dependent manner.",

keywords = "5-Hydroxytryptamine, Calcium, Glucocorticoid, Protein kinase C",

author = "Han, {Jian Zhong} and Wen Lin and Lou, {Shu Jie} and Jian Qiu and Chen, {Yi Zhang}",

note = "Funding Information: This work was supported by grants from National Basic Research Program of China (G1999054003) and Natural Science Foundation of China (Grants 39330100 and 39840019). We are also grateful to Dr. Zhuan Zhou (Institute of Neuroscience, Chinese Academy of Sciences) for providing us the superfusion apparatus from which our experiments greatly benefited.",

year = "2002",

month = aug,

day = "19",

doi = "10.1016/S0167-4889(02)00242-2",

language = "English (US)",

volume = "1591",

pages = "21--27",

journal = "Biochimica et Biophysica Acta - Molecular Cell Research",

issn = "0167-4889",

publisher = "Elsevier",

number = "1-3",

}

TY - JOUR

T1 - A rapid, nongenomic action of glucocorticoids in rat B103 neuroblastoma cells

AU - Han, Jian Zhong

AU - Lin, Wen

AU - Lou, Shu Jie

AU - Qiu, Jian

AU - Chen, Yi Zhang

N1 - Funding Information: This work was supported by grants from National Basic Research Program of China (G1999054003) and Natural Science Foundation of China (Grants 39330100 and 39840019). We are also grateful to Dr. Zhuan Zhou (Institute of Neuroscience, Chinese Academy of Sciences) for providing us the superfusion apparatus from which our experiments greatly benefited.

PY - 2002/8/19

Y1 - 2002/8/19

N2 - We report here a new example in which glucocorticoids (GCs) acted in a rapid, nongenomic way. In rat B103 neuroblastoma cells, 5-hydroxytryptamine (5-HT) was found to evoke an immediate rise in intracellular free calcium concentration ([Ca2+]i). Pre-incubation of B103 cells for 5 min with corticosterone (B) or bovine serum albumin-conjugated corticosterone (B-BSA) concentration-dependently (10-4-10-8 M) inhibited the peak increments in [Ca2+]i. Cortisol and dexamethasone had a similar effect, while deoxycorticosterone and cholesterol were ineffective. This rapid inhibitory effect of corticosterone could be mimicked by protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) and abolished completely by PKC inhibitors Ro31-8220 or GF-109203X. Neither pertussis toxin (PTX) nor nuclear GC receptor (GR) antagonist RU38486 influenced the rapid action of B. Our results suggest that GCs can modulate the 5-HT-induced Ca2+ response in B103 cells in a membrane-initiated, nongenomic, and PKC-dependent manner.

AB - We report here a new example in which glucocorticoids (GCs) acted in a rapid, nongenomic way. In rat B103 neuroblastoma cells, 5-hydroxytryptamine (5-HT) was found to evoke an immediate rise in intracellular free calcium concentration ([Ca2+]i). Pre-incubation of B103 cells for 5 min with corticosterone (B) or bovine serum albumin-conjugated corticosterone (B-BSA) concentration-dependently (10-4-10-8 M) inhibited the peak increments in [Ca2+]i. Cortisol and dexamethasone had a similar effect, while deoxycorticosterone and cholesterol were ineffective. This rapid inhibitory effect of corticosterone could be mimicked by protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) and abolished completely by PKC inhibitors Ro31-8220 or GF-109203X. Neither pertussis toxin (PTX) nor nuclear GC receptor (GR) antagonist RU38486 influenced the rapid action of B. Our results suggest that GCs can modulate the 5-HT-induced Ca2+ response in B103 cells in a membrane-initiated, nongenomic, and PKC-dependent manner.

KW - 5-Hydroxytryptamine

KW - Calcium

KW - Glucocorticoid

KW - Protein kinase C

UR - http://www.scopus.com/inward/record.url?scp=0037135717&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037135717&partnerID=8YFLogxK

U2 - 10.1016/S0167-4889(02)00242-2

DO - 10.1016/S0167-4889(02)00242-2

M3 - Article

C2 - 12183051

AN - SCOPUS:0037135717

SN - 0167-4889

VL - 1591

SP - 21

EP - 27

JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

IS - 1-3

ER -

A rapid, nongenomic action of glucocorticoids in rat B103 neuroblastoma cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this