A ribose modification of Spinach aptamer accelerates lead(ii) cation association: In vitro

Jonathan C. Savage; Pushkar Shinde; Hans Peter Bächinger; Monika A. Davare; Ujwal Shinde

doi:10.1039/c9cc01697j

A ribose modification of Spinach aptamer accelerates lead(ii) cation association: In vitro

Jonathan C. Savage, Pushkar Shinde, Hans Peter Bächinger, Monika A. Davare, Ujwal Shinde

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Spinach aptamer fluorescence requires formation of a tripartite complex composed of folded RNA, a GFP-like fluorophore, and selective cation coordination. 2′F pyrimidine modified Spinach has retained fluorescence, increased chemical stability, and accelerated cation association via increased G-quadruplex dynamics, thereby reducing readout time and enhancing Spinach utility for aqueous Pb²⁺ detection.

Original language	English (US)
Pages (from-to)	5882-5885
Number of pages	4
Journal	Chemical Communications
Volume	55
Issue number	42
DOIs	https://doi.org/10.1039/c9cc01697j
State	Published - 2019

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

Access to Document

10.1039/c9cc01697j

Cite this

@article{98ea57197a3241849b0815aeb1e0f4e8,

title = "A ribose modification of Spinach aptamer accelerates lead(ii) cation association: In vitro",

abstract = "Spinach aptamer fluorescence requires formation of a tripartite complex composed of folded RNA, a GFP-like fluorophore, and selective cation coordination. 2′F pyrimidine modified Spinach has retained fluorescence, increased chemical stability, and accelerated cation association via increased G-quadruplex dynamics, thereby reducing readout time and enhancing Spinach utility for aqueous Pb2+ detection.",

author = "Savage, {Jonathan C.} and Pushkar Shinde and B{\"a}chinger, {Hans Peter} and Davare, {Monika A.} and Ujwal Shinde",

note = "Funding Information: We would like to thank Dr David Farrens for the use of his spectroscopic equipment, and Drs Phil Yates and Omar Davulcu for their thoughtful discussion. This research was supported by the Medical Research Foundation of Oregon (GBIMO0231A; U. S.), the St. Baldrick{\textquoteright}s Foundation (GPEDI1059A; M. A. D.) and the NIH (5T32AI007472-24; J. C. S). Publisher Copyright: {\textcopyright} 2019 The Royal Society of Chemistry.",

year = "2019",

doi = "10.1039/c9cc01697j",

language = "English (US)",

volume = "55",

pages = "5882--5885",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "42",

}

TY - JOUR

T1 - A ribose modification of Spinach aptamer accelerates lead(ii) cation association

T2 - In vitro

AU - Savage, Jonathan C.

AU - Shinde, Pushkar

AU - Bächinger, Hans Peter

AU - Davare, Monika A.

AU - Shinde, Ujwal

N1 - Funding Information: We would like to thank Dr David Farrens for the use of his spectroscopic equipment, and Drs Phil Yates and Omar Davulcu for their thoughtful discussion. This research was supported by the Medical Research Foundation of Oregon (GBIMO0231A; U. S.), the St. Baldrick’s Foundation (GPEDI1059A; M. A. D.) and the NIH (5T32AI007472-24; J. C. S). Publisher Copyright: © 2019 The Royal Society of Chemistry.

PY - 2019

Y1 - 2019

N2 - Spinach aptamer fluorescence requires formation of a tripartite complex composed of folded RNA, a GFP-like fluorophore, and selective cation coordination. 2′F pyrimidine modified Spinach has retained fluorescence, increased chemical stability, and accelerated cation association via increased G-quadruplex dynamics, thereby reducing readout time and enhancing Spinach utility for aqueous Pb2+ detection.

AB - Spinach aptamer fluorescence requires formation of a tripartite complex composed of folded RNA, a GFP-like fluorophore, and selective cation coordination. 2′F pyrimidine modified Spinach has retained fluorescence, increased chemical stability, and accelerated cation association via increased G-quadruplex dynamics, thereby reducing readout time and enhancing Spinach utility for aqueous Pb2+ detection.

UR - http://www.scopus.com/inward/record.url?scp=85065992565&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85065992565&partnerID=8YFLogxK

U2 - 10.1039/c9cc01697j

DO - 10.1039/c9cc01697j

M3 - Article

C2 - 31037281

AN - SCOPUS:85065992565

SN - 1359-7345

VL - 55

SP - 5882

EP - 5885

JO - Chemical Communications

JF - Chemical Communications

IS - 42

ER -

A ribose modification of Spinach aptamer accelerates lead(ii) cation association: In vitro

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this