A SNARE required for retrograde transport to the endoplasmic reticulum

Lena Burri; Oleg Varlamov; Claudia A. Doege; Kay Hofmann; Traude Beilharz; James E. Rothman; Thomas H. Söllner; Trevor Lithgow

doi:10.1073/pnas.1734000100

A SNARE required for retrograde transport to the endoplasmic reticulum

Lena Burri, Oleg Varlamov, Claudia A. Doege, Kay Hofmann, Traude Beilharz, James E. Rothman, Thomas H. Söllner, Trevor Lithgow

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) are central components of the machinery mediating membrane fusion in all eukaryotic cells. Sequence analysis of the yeast genome revealed a previously uncharacterized SNARE, SNARE-/ike tail-anchored protein 1 (Slt1). Slt1 is an essential protein localized in the endoplasmic reticulum (ER). It forms a SNARE complex with Sec22 and the ER syntaxin Ufe1. Down-regulation of Slt1 levels leads to improper secretion of proteins normally resident in the ER. We suggest that Slt1 is a component of the SNAREpin required for retrograde traffic to the ER. Based on the previously reported association with Ufe1 and Sec22, Sec20 likely contributes the fourth SNARE to the SNAREpin.

Original language	English (US)
Pages (from-to)	9873-9877
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	100
Issue number	17
DOIs	https://doi.org/10.1073/pnas.1734000100
State	Published - Aug 19 2003
Externally published	Yes

Keywords

Membrane fusion
Secretion
Slt1
Tail-anchored protein

ASJC Scopus subject areas

General

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10.1073/pnas.1734000100

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title = "A SNARE required for retrograde transport to the endoplasmic reticulum",

abstract = "SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) are central components of the machinery mediating membrane fusion in all eukaryotic cells. Sequence analysis of the yeast genome revealed a previously uncharacterized SNARE, SNARE-/ike tail-anchored protein 1 (Slt1). Slt1 is an essential protein localized in the endoplasmic reticulum (ER). It forms a SNARE complex with Sec22 and the ER syntaxin Ufe1. Down-regulation of Slt1 levels leads to improper secretion of proteins normally resident in the ER. We suggest that Slt1 is a component of the SNAREpin required for retrograde traffic to the ER. Based on the previously reported association with Ufe1 and Sec22, Sec20 likely contributes the fourth SNARE to the SNAREpin.",

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T1 - A SNARE required for retrograde transport to the endoplasmic reticulum

AU - Burri, Lena

AU - Varlamov, Oleg

AU - Doege, Claudia A.

AU - Hofmann, Kay

AU - Beilharz, Traude

AU - Rothman, James E.

AU - Söllner, Thomas H.

AU - Lithgow, Trevor

PY - 2003/8/19

Y1 - 2003/8/19

N2 - SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) are central components of the machinery mediating membrane fusion in all eukaryotic cells. Sequence analysis of the yeast genome revealed a previously uncharacterized SNARE, SNARE-/ike tail-anchored protein 1 (Slt1). Slt1 is an essential protein localized in the endoplasmic reticulum (ER). It forms a SNARE complex with Sec22 and the ER syntaxin Ufe1. Down-regulation of Slt1 levels leads to improper secretion of proteins normally resident in the ER. We suggest that Slt1 is a component of the SNAREpin required for retrograde traffic to the ER. Based on the previously reported association with Ufe1 and Sec22, Sec20 likely contributes the fourth SNARE to the SNAREpin.

AB - SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) are central components of the machinery mediating membrane fusion in all eukaryotic cells. Sequence analysis of the yeast genome revealed a previously uncharacterized SNARE, SNARE-/ike tail-anchored protein 1 (Slt1). Slt1 is an essential protein localized in the endoplasmic reticulum (ER). It forms a SNARE complex with Sec22 and the ER syntaxin Ufe1. Down-regulation of Slt1 levels leads to improper secretion of proteins normally resident in the ER. We suggest that Slt1 is a component of the SNAREpin required for retrograde traffic to the ER. Based on the previously reported association with Ufe1 and Sec22, Sec20 likely contributes the fourth SNARE to the SNAREpin.

KW - Membrane fusion

KW - Secretion

KW - Slt1

KW - Tail-anchored protein

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ER -

A SNARE required for retrograde transport to the endoplasmic reticulum

Abstract

Keywords

ASJC Scopus subject areas

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