TY - JOUR
T1 - A solid-phase assay for circulating immune complexes using monoclonal rheumatoid factors and peroxidase-linked protein A
AU - Davey, Michael P.
AU - Korngold, Leonhard
N1 - Funding Information:
In the present paper we describe a new solid-phase mRF assay for IC based on the enzyme-linked immunosorbent assay (ELISA). This assay can be used in a clinical laboratory for the detection of circulating IC; it is simple, inexpensive, reproducible and requires no radioisotopes or unusual I This study was supported by Biomedical Research Support Grant RR-05495, Division of Research Resources, National Institutes of Health. 2 Work performed in partial fulfillment of the requirements of the doctoral dissertation. 3 Mailing address: Dr. Leonhard Korngold, Hospital for Special Surgery, 535 East 70th Street, New York, NY 10021, U.S.A.
PY - 1981/7/17
Y1 - 1981/7/17
N2 - A solid-phase monoclonal rheumatoid factor (mRF) assay for circulating immune complexes (IC) is described. Microtiter plates are coated with mRF, serum diluted 1 : 4 is added, and complexed IgG is detected with protein A coupled to peroxidase. Selective binding was obtained with aggregated human gamma-globulin and soluble IC prepared in vitro. Sucrose density gradient ultracentrifugation studies indicate that mRF binds intermediate-size IC (between 7S and 19S). Abnormal levels of IC were found in 78% of sera from patients with rheumatoid arthritis, 67% with systemic lupus erythematosus, and 87% with subacute bacterial endocarditis.
AB - A solid-phase monoclonal rheumatoid factor (mRF) assay for circulating immune complexes (IC) is described. Microtiter plates are coated with mRF, serum diluted 1 : 4 is added, and complexed IgG is detected with protein A coupled to peroxidase. Selective binding was obtained with aggregated human gamma-globulin and soluble IC prepared in vitro. Sucrose density gradient ultracentrifugation studies indicate that mRF binds intermediate-size IC (between 7S and 19S). Abnormal levels of IC were found in 78% of sera from patients with rheumatoid arthritis, 67% with systemic lupus erythematosus, and 87% with subacute bacterial endocarditis.
UR - http://www.scopus.com/inward/record.url?scp=0019500131&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019500131&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(81)90110-1
DO - 10.1016/0022-1759(81)90110-1
M3 - Article
C2 - 7252177
AN - SCOPUS:0019500131
SN - 0022-1759
VL - 44
SP - 87
EP - 100
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1
ER -