@inbook{0c4fc6a687c74c719def2725c06be897,
title = "A suite of bioassays to evaluate CREB inhibitors",
abstract = "The cyclic-AMP response element binding protein (CREB) is an important nuclear transcription factor and has been shown to be overexpressed and/or over-activated in many different cancer types, suggesting that targeting CREB is a novel approach for developing cancer therapies. Our lab discovered the first cell-permeable small molecule inhibitor of CREB, from which we further developed a potent CREB inhibitor with in vivo anti-cancer activity. In this article, we detailed our biochemical and cell-based bioassays to assess different small molecule CREB inhibitors.",
keywords = "CREB, Inhibitor, Luciferase, Protein-protein interaction, Reporter, Transcription",
author = "Li, {Bingbing X.} and Xiangshu Xiao",
note = "Funding Information: This work was supported by NIH R01GM122820, R21CA220061, Oregon Health & Science University School of Medicine, Office of the Technology Transfer and Business Development, Oregon Clinical and Translational Research Institute, Medical Research Foundation of Oregon and Susan G. Komen for the Cure (KG100458). We thank Prof. Tom Kodadek (Scripps Florida) for sharing CRE-FLuc plasmid and Prof. Sanjiv Gambhir (Stanford University) for sharing Id-RLucN plasmid. Funding Information: This work was supported by NIH R01GM122820, R21CA220061, Oregon Health & Science University School of Medicine, Office of the Technology Transfer and Business Development, Oregon Clinical and Translational Research Institute, Medical Research Foundation of Oregon and Susan G. Komen for the Cure (KG100458). We thank Prof. Tom Kodadek (Scripps Florida) for sharing CRE-FLuc plasmid and Prof. Sanjiv Gambhir (Stanford University) for sharing Id-RLucN plasmid. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
doi = "10.1016/bs.mie.2019.11.002",
language = "English (US)",
isbn = "9780128191286",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "169--184",
editor = "Shukla, {Arun K.}",
booktitle = "Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Part C",
}