TY - JOUR
T1 - A Toolkit to Quantify Target Compounds in Thin-Layer-Chromatography Experiments
AU - Mac Fhionnlaoich, Niamh
AU - Ibsen, Stuart
AU - Serrano, Luis A.
AU - Taylor, Alaric
AU - Qi, Runzhang
AU - Guldin, Stefan
N1 - Funding Information:
This project received funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement no. 633635 (DIACHEMO). N.M.F. is grateful for support by the EPSRC under a Doctoral Training Partnership (EP/M507970/1). The authors acknowledge valuable feedback from Jin Hao and Wei Lik (University College London).
Publisher Copyright:
Copyright © 2018 American Chemical Society and Division of Chemical Education, Inc.
PY - 2018/12/11
Y1 - 2018/12/11
N2 - Thin-layer chromatography (TLC) is one of the basic analytical procedures in chemistry and allows the demonstration of various chemical principles in an educational setting. An often-overlooked aspect of TLC is the capability to quantify isolated target compounds in an unknown sample. Here, we present a suitable route to implement quantitative analysis in a lesson plan. We provide both a stand-alone software and an online webapp that allow students to obtain quantitative information from a developed TLC plate and present two suitable experiments, namely, the absorbance-based quantification of the colorant Sudan IV and the fluorescence-based quantification of rhodamine 6G, a fluorophore widely used in biotechnology. Students conduct TLC experiments following established protocols, take pictures of their TLC plates with mobile phones, and subsequently quantify the different compounds in the separate bands they observe.
AB - Thin-layer chromatography (TLC) is one of the basic analytical procedures in chemistry and allows the demonstration of various chemical principles in an educational setting. An often-overlooked aspect of TLC is the capability to quantify isolated target compounds in an unknown sample. Here, we present a suitable route to implement quantitative analysis in a lesson plan. We provide both a stand-alone software and an online webapp that allow students to obtain quantitative information from a developed TLC plate and present two suitable experiments, namely, the absorbance-based quantification of the colorant Sudan IV and the fluorescence-based quantification of rhodamine 6G, a fluorophore widely used in biotechnology. Students conduct TLC experiments following established protocols, take pictures of their TLC plates with mobile phones, and subsequently quantify the different compounds in the separate bands they observe.
KW - Analytical Chemistry
KW - Computer-Based Learning
KW - Continuing Education
KW - Demonstrations
KW - Environmental Chemistry
KW - First-Year Undergraduate/General
KW - General Public
KW - Graduate Education/Research
KW - Hands-On Learning/Manipulatives
KW - High School/Introductory Chemistry
KW - Inquiry-Based/Discovery Learning
KW - Laboratory Computing/Interfacing
KW - Laboratory Equipment/Apparatus
KW - Laboratory Instruction
KW - Microscale Lab
KW - Problem Solving/Decision Making
KW - Public Understanding/Outreach
KW - Quantitative Analysis
KW - Second-Year Undergraduate
KW - Student-Centered Learning
KW - Thin Layer Chromatography
KW - Upper-Division Undergraduate
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U2 - 10.1021/acs.jchemed.8b00144
DO - 10.1021/acs.jchemed.8b00144
M3 - Article
AN - SCOPUS:85055003430
SN - 0021-9584
VL - 95
SP - 2191
EP - 2196
JO - Journal of Chemical Education
JF - Journal of Chemical Education
IS - 12
ER -