TY - JOUR
T1 - ADAMTS-1/METH-1 and TIMP-3 expression in the primate corpus luteum
T2 - Divergent patterns and stage-dependent regulation during the natural menstrual cycle
AU - Young, Kelly A.
AU - Tumlinson, Barton
AU - Stouffer, Richard L.
N1 - Funding Information:
We are grateful to both Serono Reproductive Biology Institute and Sanofi Research Division for providing research materials, along with the advice and expertise from members of the Stouffer, Hennebold, and Zelinski Laboratories (Marina Peluffo, Fuhua Xu, Ted Molskness, Yibing Jia, Carol Gib-bins and Jessica Vance), and the staff of the Department of Animal Resources. This research was supported by NIH NICHD HD20869 (R.L.S.), through a cooperative agreement (U54-HD18185) as part of the Specialized Cooperative Centers Program in Reproduction Research, NCRR RR00163 (R.L.S.), The M.J.Murdock Charitable Trust (#2001279, B.T.), and NICHD NRSA HD042869 (K.A.Y.).
PY - 2004/8
Y1 - 2004/8
N2 - Studies were designed to determine if ADAMTS-1 (a disintegrin and metalloproteinase with thrombospondin repeats-1) is expressed in the rhesus monkey corpus luteum (CL), is regulated by endocrine (LH) or local (progesterone) factors, and is correlated with tissue inhibitor of matrix metalioproteinase-3 (TIMP-3), an inhibitor of ADAMTS-1. PCR analyses indicated that ADAMTS-1 mRNA is expressed in luteinized granulosa cells during controlled ovarian stimulation cycles, and peaks in CL during the early luteal phase of the menstrual cycle, before decreasing (P < 0.05) by the mid-late stage. Immunostaining for ADAMTS-1 was detected in luteal cells, peaking in early CL. LH and/or steroid depletion at mid-late luteal stage decreased (P < 0.05) ADAMTS-1 mRNA levels compared to controls; LH but not progestin (R5020) replacement prevented this decrease. In contrast, LH and/or steroid ablation-replacement in the early CL did not affect ADAMTS-1 levels. TIMP-3 mRNA levels were lowest during the early CL and rose progressively (P < 0.05), peaking in late CL. The divergent expression patterns during the CL lifespan suggest that an imbalance between ADAMTS-1 and TIMP-3 is important during luteal formation (ADAMTS-1 predominates) and regression (TIMP-3 predominates). Also, LH, perhaps via steroids other than progesterone, promotes ADAMTS-1 expression as a function of the stage of the CL.
AB - Studies were designed to determine if ADAMTS-1 (a disintegrin and metalloproteinase with thrombospondin repeats-1) is expressed in the rhesus monkey corpus luteum (CL), is regulated by endocrine (LH) or local (progesterone) factors, and is correlated with tissue inhibitor of matrix metalioproteinase-3 (TIMP-3), an inhibitor of ADAMTS-1. PCR analyses indicated that ADAMTS-1 mRNA is expressed in luteinized granulosa cells during controlled ovarian stimulation cycles, and peaks in CL during the early luteal phase of the menstrual cycle, before decreasing (P < 0.05) by the mid-late stage. Immunostaining for ADAMTS-1 was detected in luteal cells, peaking in early CL. LH and/or steroid depletion at mid-late luteal stage decreased (P < 0.05) ADAMTS-1 mRNA levels compared to controls; LH but not progestin (R5020) replacement prevented this decrease. In contrast, LH and/or steroid ablation-replacement in the early CL did not affect ADAMTS-1 levels. TIMP-3 mRNA levels were lowest during the early CL and rose progressively (P < 0.05), peaking in late CL. The divergent expression patterns during the CL lifespan suggest that an imbalance between ADAMTS-1 and TIMP-3 is important during luteal formation (ADAMTS-1 predominates) and regression (TIMP-3 predominates). Also, LH, perhaps via steroids other than progesterone, promotes ADAMTS-1 expression as a function of the stage of the CL.
KW - ADAMTS-1
KW - Corpus luteum
KW - Ovary
KW - Primate
KW - TIMP-3
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U2 - 10.1093/molehr/gah079
DO - 10.1093/molehr/gah079
M3 - Article
C2 - 15208368
AN - SCOPUS:4344639838
SN - 1360-9947
VL - 10
SP - 559
EP - 565
JO - Molecular Human Reproduction
JF - Molecular Human Reproduction
IS - 8
ER -