@article{23bcd1cf739c4990a3776828fd831e52,
title = "Advances in structural and functional analysis of membrane proteins by electron crystallography",
abstract = "Electron crystallography is a powerful technique for the study of membrane protein structure and function in the lipid environment. When well-ordered two-dimensional crystals are obtained the structure of both protein and lipid can be determined and lipid-protein interactions analyzed. Protons and ionic charges can be visualized by electron crystallography and the protein of interest can be captured for structural analysis in a variety of physiologically distinct states. This review highlights the strengths of electron crystallography and the momentum that is building up in automation and the development of high throughput tools and methods for structural and functional analysis of membrane proteins by electron crystallography.",
author = "Goragot Wisedchaisri and Reichow, {Steve L.} and Tamir Gonen",
note = "Funding Information: We thank Drs. Anchi Cheng (The Scripps Research Institute, La Jolla, USA), Andreas Engel (Case Western Reserve University, Cleveland, USA), Yoshinori Fujiyoshi (Kyoto University, Kyoto, Japan), David Stokes (New York University, New York, USA), and Iban Ubarretxena-Belandia (Mount Sinai School of Medicine, New York, USA) for providing us with high-resolution images of their work for inclusion in this review. We thank Yoshinori Fujiyoshi, Kazutoshi Tani (Kyoto University, Japan), and Kaoru Mitsuoka (Biomedicinal Information Research Center [BIRC] Tokyo, Japan) for kindly providing the data sets for aquaporin-4 and bacteriorhodopsin; and to Dr. Sriram Subramaniam (National Cancer Institute, Bethesda, USA) for sending us PDB coordinate files for the oxalate transporter. S.L.R. is supported by the National Institutes for Health Ruth L. Kirschstein National Research Service Award (EY19768). The Gonen laboratory is supported by the National Institutes of Health (GM079233 and U54GM094598); the American Diabetes Association (1-09-CD-05) and by the Howard Hughes Medical Institute.",
year = "2011",
month = oct,
day = "12",
doi = "10.1016/j.str.2011.09.001",
language = "English (US)",
volume = "19",
pages = "1381--1393",
journal = "Structure",
issn = "0969-2126",
publisher = "Cell Press",
number = "10",
}