Age-related cochlear cytokine gene expression in the BALB/cJ mouse with systemic versus intratympanic dosing of steroid drugs

Sara A. Tokarz; Jiaqing Pang; Anna Grosz; J. Beth Kempton; Dennis R. Trune; De Ann M. Pillers

doi:10.3109/00016489.2013.771407

Age-related cochlear cytokine gene expression in the BALB/cJ mouse with systemic versus intratympanic dosing of steroid drugs

Sara A. Tokarz, Jiaqing Pang, Anna Grosz, J. Beth Kempton, Dennis R. Trune, De Ann M. Pillers

Otolaryngology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Conclusion: Age-related differences in the expression of inflammatory cytokines in the inner ear may contribute to the development of age-related hearing loss (ARHL). Objectives: ARHL is characterized by tissue remodeling, ischemia, ion homeostasis, and inflammation. Steroid therapy is an otoprotective strategy that likely acts by reducing inflammation. We examined age-related changes in cytokine gene expression in the cochlea of the BALB/cJ mouse model of premature ARHL after systemic or intratympanic steroid delivery. Methods: 'Young' (2.5-3 months) and 'Old' (5-9 months) mice were treated with dexamethasone or fludrocortisone administered either orally or intratympanically. Cytokine gene expression in cochlear RNA was analyzed using prefabricated cDNA arrays. Old groups were compared to Young groups to identify age-related changes. Results: Down-regulation of a cytokine associated with bone remodeling (SPP1) was observed in the untreated Old group. Numerous genes were up-or down-regulated by more than twofold by steroid treatment, including proinflammatory interleukins (IL-16) and anti-inflammatory cytokines.

Original language	English (US)
Pages (from-to)	685-691
Number of pages	7
Journal	Acta Oto-Laryngologica
Volume	133
Issue number	7
DOIs	https://doi.org/10.3109/00016489.2013.771407
State	Published - Jul 2013

Keywords

Aging
Animal model
Glucocorticoid
Inflammation
Inner ear
Mineralocorticoid
SPP1
Sensorineural hearing loss

ASJC Scopus subject areas

Otorhinolaryngology

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10.3109/00016489.2013.771407

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@article{002d4d1d673b477b8bce07b466bc1adc,

title = "Age-related cochlear cytokine gene expression in the BALB/cJ mouse with systemic versus intratympanic dosing of steroid drugs",

abstract = "Conclusion: Age-related differences in the expression of inflammatory cytokines in the inner ear may contribute to the development of age-related hearing loss (ARHL). Objectives: ARHL is characterized by tissue remodeling, ischemia, ion homeostasis, and inflammation. Steroid therapy is an otoprotective strategy that likely acts by reducing inflammation. We examined age-related changes in cytokine gene expression in the cochlea of the BALB/cJ mouse model of premature ARHL after systemic or intratympanic steroid delivery. Methods: 'Young' (2.5-3 months) and 'Old' (5-9 months) mice were treated with dexamethasone or fludrocortisone administered either orally or intratympanically. Cytokine gene expression in cochlear RNA was analyzed using prefabricated cDNA arrays. Old groups were compared to Young groups to identify age-related changes. Results: Down-regulation of a cytokine associated with bone remodeling (SPP1) was observed in the untreated Old group. Numerous genes were up-or down-regulated by more than twofold by steroid treatment, including proinflammatory interleukins (IL-16) and anti-inflammatory cytokines.",

keywords = "Aging, Animal model, Glucocorticoid, Inflammation, Inner ear, Mineralocorticoid, SPP1, Sensorineural hearing loss",

author = "Tokarz, {Sara A.} and Jiaqing Pang and Anna Grosz and {Beth Kempton}, J. and Trune, {Dennis R.} and Pillers, {De Ann M.}",

note = "Funding Information: We thank Jens Eickhoff PhD, University of Wisconsin-Madison Department of Biostatistics & Medical Informatics, for helpful review of the statistics applied in this manuscript. This work was funded through NIH grant NIH-NIDCD R01 DC05593 (D.R.T.), the Doernbecher Foundation (D.M.P.), the OHSU Department of Pediatrics (D.M.P.), and the University of Wisconsin-Madison Department of Pediatrics (D.M.P.).",

year = "2013",

month = jul,

doi = "10.3109/00016489.2013.771407",

language = "English (US)",

volume = "133",

pages = "685--691",

journal = "Acta Oto-Laryngologica",

issn = "0001-6489",

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T1 - Age-related cochlear cytokine gene expression in the BALB/cJ mouse with systemic versus intratympanic dosing of steroid drugs

AU - Tokarz, Sara A.

AU - Pang, Jiaqing

AU - Grosz, Anna

AU - Beth Kempton, J.

AU - Trune, Dennis R.

AU - Pillers, De Ann M.

N1 - Funding Information: We thank Jens Eickhoff PhD, University of Wisconsin-Madison Department of Biostatistics & Medical Informatics, for helpful review of the statistics applied in this manuscript. This work was funded through NIH grant NIH-NIDCD R01 DC05593 (D.R.T.), the Doernbecher Foundation (D.M.P.), the OHSU Department of Pediatrics (D.M.P.), and the University of Wisconsin-Madison Department of Pediatrics (D.M.P.).

PY - 2013/7

Y1 - 2013/7

N2 - Conclusion: Age-related differences in the expression of inflammatory cytokines in the inner ear may contribute to the development of age-related hearing loss (ARHL). Objectives: ARHL is characterized by tissue remodeling, ischemia, ion homeostasis, and inflammation. Steroid therapy is an otoprotective strategy that likely acts by reducing inflammation. We examined age-related changes in cytokine gene expression in the cochlea of the BALB/cJ mouse model of premature ARHL after systemic or intratympanic steroid delivery. Methods: 'Young' (2.5-3 months) and 'Old' (5-9 months) mice were treated with dexamethasone or fludrocortisone administered either orally or intratympanically. Cytokine gene expression in cochlear RNA was analyzed using prefabricated cDNA arrays. Old groups were compared to Young groups to identify age-related changes. Results: Down-regulation of a cytokine associated with bone remodeling (SPP1) was observed in the untreated Old group. Numerous genes were up-or down-regulated by more than twofold by steroid treatment, including proinflammatory interleukins (IL-16) and anti-inflammatory cytokines.

AB - Conclusion: Age-related differences in the expression of inflammatory cytokines in the inner ear may contribute to the development of age-related hearing loss (ARHL). Objectives: ARHL is characterized by tissue remodeling, ischemia, ion homeostasis, and inflammation. Steroid therapy is an otoprotective strategy that likely acts by reducing inflammation. We examined age-related changes in cytokine gene expression in the cochlea of the BALB/cJ mouse model of premature ARHL after systemic or intratympanic steroid delivery. Methods: 'Young' (2.5-3 months) and 'Old' (5-9 months) mice were treated with dexamethasone or fludrocortisone administered either orally or intratympanically. Cytokine gene expression in cochlear RNA was analyzed using prefabricated cDNA arrays. Old groups were compared to Young groups to identify age-related changes. Results: Down-regulation of a cytokine associated with bone remodeling (SPP1) was observed in the untreated Old group. Numerous genes were up-or down-regulated by more than twofold by steroid treatment, including proinflammatory interleukins (IL-16) and anti-inflammatory cytokines.

KW - Aging

KW - Animal model

KW - Glucocorticoid

KW - Inflammation

KW - Inner ear

KW - Mineralocorticoid

KW - SPP1

KW - Sensorineural hearing loss

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ER -

Age-related cochlear cytokine gene expression in the BALB/cJ mouse with systemic versus intratympanic dosing of steroid drugs

Abstract

Keywords

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