Analysis of IL-17 production by flow cytometry and ELISPOT assays

Ling Zhao, Yuan Chou, Yanfang Jiang, Zhenyu Jiang, Cong Qiu Chu

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Interleukin (IL)-17 represents a family of cytokines with six members, namely IL-17A, B, C, D, E, and F. IL-17A and IL-17F are best studied proinflammatory cytokines. CD4+ T helper cells producing IL-17A have been identified as a distinct T helper subset, Th17 cells. IL-17 and Th17 cells are important mediators in tissue inflammation in immune-mediated inflammatory diseases. IL-17 is also produced by other immune cells and plays an important role in host defense against microbial infection. Cell-based assays are sensitive and quantitative, and enable identification of cellular sources of IL-17 production. This chapter describes usage of flow cytometry and ELISPOT assays to quantify IL-17A-producing cells in disease and in vitro experiments to study T cell function.

Original languageEnglish (US)
Pages (from-to)243-256
Number of pages14
JournalMethods in Molecular Biology
StatePublished - 2014
Externally publishedYes


  • ELISPOT assay
  • Flow cytometry
  • Inflammation
  • Interleukin-17
  • Th17 cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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