Analysis of IL-17 production by flow cytometry and ELISPOT assays

Ling Zhao; Yuan Chou; Yanfang Jiang; Zhenyu Jiang; Cong Qiu Chu

doi:10.1007/978-1-4939-0928-5_22

Analysis of IL-17 production by flow cytometry and ELISPOT assays

Ling Zhao, Yuan Chou, Yanfang Jiang, Zhenyu Jiang, Cong Qiu Chu

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Interleukin (IL)-17 represents a family of cytokines with six members, namely IL-17A, B, C, D, E, and F. IL-17A and IL-17F are best studied proinflammatory cytokines. CD4⁺ T helper cells producing IL-17A have been identified as a distinct T helper subset, Th17 cells. IL-17 and Th17 cells are important mediators in tissue inflammation in immune-mediated inflammatory diseases. IL-17 is also produced by other immune cells and plays an important role in host defense against microbial infection. Cell-based assays are sensitive and quantitative, and enable identification of cellular sources of IL-17 production. This chapter describes usage of flow cytometry and ELISPOT assays to quantify IL-17A-producing cells in disease and in vitro experiments to study T cell function.

Original language	English (US)
Pages (from-to)	243-256
Number of pages	14
Journal	Methods in Molecular Biology
Volume	1172
DOIs	https://doi.org/10.1007/978-1-4939-0928-5_22
State	Published - 2014
Externally published	Yes

Keywords

ELISPOT assay
Flow cytometry
Inflammation
Interleukin-17
Th17 cells

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-0928-5_22

Cite this

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title = "Analysis of IL-17 production by flow cytometry and ELISPOT assays",

abstract = "Interleukin (IL)-17 represents a family of cytokines with six members, namely IL-17A, B, C, D, E, and F. IL-17A and IL-17F are best studied proinflammatory cytokines. CD4+ T helper cells producing IL-17A have been identified as a distinct T helper subset, Th17 cells. IL-17 and Th17 cells are important mediators in tissue inflammation in immune-mediated inflammatory diseases. IL-17 is also produced by other immune cells and plays an important role in host defense against microbial infection. Cell-based assays are sensitive and quantitative, and enable identification of cellular sources of IL-17 production. This chapter describes usage of flow cytometry and ELISPOT assays to quantify IL-17A-producing cells in disease and in vitro experiments to study T cell function.",

keywords = "ELISPOT assay, Flow cytometry, Inflammation, Interleukin-17, Th17 cells",

author = "Ling Zhao and Yuan Chou and Yanfang Jiang and Zhenyu Jiang and Chu, {Cong Qiu}",

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year = "2014",

doi = "10.1007/978-1-4939-0928-5_22",

language = "English (US)",

volume = "1172",

pages = "243--256",

journal = "Methods in Molecular Biology",

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T1 - Analysis of IL-17 production by flow cytometry and ELISPOT assays

AU - Zhao, Ling

AU - Chou, Yuan

AU - Jiang, Yanfang

AU - Jiang, Zhenyu

AU - Chu, Cong Qiu

N1 - Publisher Copyright: © Springer Science+Business Media New York 2014.

PY - 2014

Y1 - 2014

N2 - Interleukin (IL)-17 represents a family of cytokines with six members, namely IL-17A, B, C, D, E, and F. IL-17A and IL-17F are best studied proinflammatory cytokines. CD4+ T helper cells producing IL-17A have been identified as a distinct T helper subset, Th17 cells. IL-17 and Th17 cells are important mediators in tissue inflammation in immune-mediated inflammatory diseases. IL-17 is also produced by other immune cells and plays an important role in host defense against microbial infection. Cell-based assays are sensitive and quantitative, and enable identification of cellular sources of IL-17 production. This chapter describes usage of flow cytometry and ELISPOT assays to quantify IL-17A-producing cells in disease and in vitro experiments to study T cell function.

AB - Interleukin (IL)-17 represents a family of cytokines with six members, namely IL-17A, B, C, D, E, and F. IL-17A and IL-17F are best studied proinflammatory cytokines. CD4+ T helper cells producing IL-17A have been identified as a distinct T helper subset, Th17 cells. IL-17 and Th17 cells are important mediators in tissue inflammation in immune-mediated inflammatory diseases. IL-17 is also produced by other immune cells and plays an important role in host defense against microbial infection. Cell-based assays are sensitive and quantitative, and enable identification of cellular sources of IL-17 production. This chapter describes usage of flow cytometry and ELISPOT assays to quantify IL-17A-producing cells in disease and in vitro experiments to study T cell function.

KW - ELISPOT assay

KW - Flow cytometry

KW - Inflammation

KW - Interleukin-17

KW - Th17 cells

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SN - 1064-3745

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SP - 243

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Analysis of IL-17 production by flow cytometry and ELISPOT assays

Abstract

Keywords

ASJC Scopus subject areas

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