Abstract
Infiltration of leukocytes into tissue is a common feature of many physiological and pathological conditions. Histopathologically, the diversity of leukocytes that infiltrate a tissue associated with a pathophysiologic response cannot be appreciated and/or examined unless highly selective immunologic detection methods are utilized. Specific populations of infiltrating leukocytes into squamous tissues harboring pre-malignant and/or malignant keratinocytes have recently been demonstrated to play a functionally significant role in the pathogenesis of squamous carcinomas. To evaluate immune cell types and quantify changes in their relative presence and localization during multi-stage neoplastic progression, we performed flow cytometry and histochemical detection using lineage-selective markers. Herein, we provide detailed methodology facilitating these analyses.
Original language | English (US) |
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Pages (from-to) | 36-43 |
Number of pages | 8 |
Journal | Journal of Investigative Dermatology Symposium Proceedings |
Volume | 11 |
Issue number | 1 |
DOIs | |
State | Published - Sep 2006 |
Externally published | Yes |
ASJC Scopus subject areas
- Biotechnology
- Molecular Biology
- Dermatology
- Cell Biology