Analysis of Muscarinic Acetylcholine Receptor Expression and Function

Neil M. Nathanson; Phyllis S. Goldman; Beth A. Habecker; Jacques C. Migeon; Mary E. Morton; Kathleen M. Tietje; Chris J. van Koppen

doi:10.1016/B978-0-12-185267-2.50014-3

Analysis of Muscarinic Acetylcholine Receptor Expression and Function

Neil M. Nathanson, Phyllis S. Goldman, Beth A. Habecker, Jacques C. Migeon, Mary E. Morton, Kathleen M. Tietje, Chris J. van Koppen

Research output: Chapter in Book/Report/Conference proceeding › Chapter

14 Scopus citations

Abstract

Muscarinic acetylcholine receptors (mAChRs) are members of the G proteinlinked receptor superfamily and are expressed in many cell types and tissues, including brain, peripheral nerves, cardiac and smooth muscles, and exocrine glands (1,2). Five subtypes of mAChR, encoded by distinct genes, have been identified in mammals, and homologous mAChR genes have been isolated from nonmammalian vertebrates (3) and invertebrates (4). This chapter will describe techniques we have used for the analysis of the number and ligandbinding properties of muscarinic receptors in intact cells and in membrane homogenates, the measurement of coupling of mAChR to changes in intracellular second messengers, the analysis of changes in G protein expression that may contribute to changes in mAChR-mediated signaling, and the quantitative measurement of mRNAs encoding specific subtypes of mAChR.

Original language	English (US)
Title of host publication	Methods in Neurosciences
Pages	116-134
Number of pages	19
Edition	C
DOIs	https://doi.org/10.1016/B978-0-12-185267-2.50014-3
State	Published - Jan 1 1992
Externally published	Yes

Publication series

Name	Methods in Neurosciences
Number	C
Volume	9
ISSN (Print)	1043-9471

ASJC Scopus subject areas

General Neuroscience

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title = "Analysis of Muscarinic Acetylcholine Receptor Expression and Function",

abstract = "Muscarinic acetylcholine receptors (mAChRs) are members of the G proteinlinked receptor superfamily and are expressed in many cell types and tissues, including brain, peripheral nerves, cardiac and smooth muscles, and exocrine glands (1,2). Five subtypes of mAChR, encoded by distinct genes, have been identified in mammals, and homologous mAChR genes have been isolated from nonmammalian vertebrates (3) and invertebrates (4). This chapter will describe techniques we have used for the analysis of the number and ligandbinding properties of muscarinic receptors in intact cells and in membrane homogenates, the measurement of coupling of mAChR to changes in intracellular second messengers, the analysis of changes in G protein expression that may contribute to changes in mAChR-mediated signaling, and the quantitative measurement of mRNAs encoding specific subtypes of mAChR.",

author = "Nathanson, {Neil M.} and Goldman, {Phyllis S.} and Habecker, {Beth A.} and Migeon, {Jacques C.} and Morton, {Mary E.} and Tietje, {Kathleen M.} and {van Koppen}, {Chris J.}",

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AU - Nathanson, Neil M.

AU - Goldman, Phyllis S.

AU - Habecker, Beth A.

AU - Migeon, Jacques C.

AU - Morton, Mary E.

AU - Tietje, Kathleen M.

AU - van Koppen, Chris J.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Muscarinic acetylcholine receptors (mAChRs) are members of the G proteinlinked receptor superfamily and are expressed in many cell types and tissues, including brain, peripheral nerves, cardiac and smooth muscles, and exocrine glands (1,2). Five subtypes of mAChR, encoded by distinct genes, have been identified in mammals, and homologous mAChR genes have been isolated from nonmammalian vertebrates (3) and invertebrates (4). This chapter will describe techniques we have used for the analysis of the number and ligandbinding properties of muscarinic receptors in intact cells and in membrane homogenates, the measurement of coupling of mAChR to changes in intracellular second messengers, the analysis of changes in G protein expression that may contribute to changes in mAChR-mediated signaling, and the quantitative measurement of mRNAs encoding specific subtypes of mAChR.

AB - Muscarinic acetylcholine receptors (mAChRs) are members of the G proteinlinked receptor superfamily and are expressed in many cell types and tissues, including brain, peripheral nerves, cardiac and smooth muscles, and exocrine glands (1,2). Five subtypes of mAChR, encoded by distinct genes, have been identified in mammals, and homologous mAChR genes have been isolated from nonmammalian vertebrates (3) and invertebrates (4). This chapter will describe techniques we have used for the analysis of the number and ligandbinding properties of muscarinic receptors in intact cells and in membrane homogenates, the measurement of coupling of mAChR to changes in intracellular second messengers, the analysis of changes in G protein expression that may contribute to changes in mAChR-mediated signaling, and the quantitative measurement of mRNAs encoding specific subtypes of mAChR.

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BT - Methods in Neurosciences

ER -

Analysis of Muscarinic Acetylcholine Receptor Expression and Function

Abstract

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