TY - JOUR
T1 - Antibodies to cell surface antigens detected by a rapid spot test using ethidium bromide
AU - Cohn, Marjorie L.
AU - Golding, Hana
AU - Rittenberg, Marvin B.
N1 - Funding Information:
This work was supported by Grant AI 14985 from the National Institutes of Health, March of Dimes Basic Research Grant 1-633, and American Cancer Society Grant IM-172. M.L.C. and H.G. are Fellows of the N.L. Tartar Foundation.
PY - 1982/2/26
Y1 - 1982/2/26
N2 - We describe here a spot test to detect antibodies to non-erythroid cell surface antigens, using ethidium bromide staining to visualize areas of target cell lysis. This assay allows rapid screening of large numbers of samples of culture supernatants, antisera, or ascitic fluids. Antibody titers determined by this spot test are shown to correlate well with titers determined by two other methods, providing a simple method for initial estimation of the amount of antibody in positive samples.
AB - We describe here a spot test to detect antibodies to non-erythroid cell surface antigens, using ethidium bromide staining to visualize areas of target cell lysis. This assay allows rapid screening of large numbers of samples of culture supernatants, antisera, or ascitic fluids. Antibody titers determined by this spot test are shown to correlate well with titers determined by two other methods, providing a simple method for initial estimation of the amount of antibody in positive samples.
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U2 - 10.1016/0022-1759(82)90367-2
DO - 10.1016/0022-1759(82)90367-2
M3 - Article
C2 - 6174640
AN - SCOPUS:0020077965
SN - 0022-1759
VL - 49
SP - 75
EP - 82
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1
ER -