TY - JOUR
T1 - Antigenic proteins associated with calcareous corpuscules of Taenia solium
T2 - Partial characterization of a calcium-binding protein
AU - Zurabian, Rimma
AU - Carrero, Julio C.
AU - Rodríguez-Contreras, Dayana
AU - Willms, Kaethe
AU - Laclette, Juan P.
N1 - Funding Information:
We thank A. Zaha for kindly providing the EgCaBP1 DNA probe and αEgCaBP1 mAb and D. Correa for the patient serum samples, P. de la Torre for DNA sequencing, F. Mendlovic for providing recombinant calreticulin and B. Sánchez-Hernández for assistance with the statistical analysis. This work was supported in part by grants from CONACYT, 41693-M (JPL, JCC); DGAPA-UNAM, IN-206102-3 (JPL, JCC) and IN238602-3 (KW); and Fundación Miguel Alemán, A.C. (JPL).
PY - 2005/1
Y1 - 2005/1
N2 - Background. A protein fraction was isolated from calcareous corpuscles of Taenia solium cysticerci. The antigens in this fraction were recognized in ELISA and Western blot assays by all sera from a group of patients with active neurocysticercosis (NC) and were not recognized by the sera from patients with other neurological disorders. Western blot analysis also showed that several high molecular weight proteins were strongly recognized by antibodies in all the neurocysticercotic patient sera, suggesting a potential for serological diagnosis of neurocysticercosis. Methods. In order to characterize these antigenic proteins, we used a monoclonal antibody raised against a high MW calcium-binding protein associated with calcareous corpuscles of Echinococcus granulosus (EgCaBP1). Results. Western blot assays revealed the recognition of a protein band of about 260 kDa, appearing within the range of the high MW antigens recognized by the NC sera. Several cDNA clones were isolated through screening of a T. solium metacestode library with a DNA probe for EgCaBP1, containing partial coding sequences showing about 88% identity with the protein of E. granulosus. Moreover, a recombinant product expressed in bacteria from the partial coding sequence of T. solium showed the ability to bind Ca2+ and was recognized by the monoclonal antibody. This recombinant calcium-binding protein of T. solium was not recognized by the NC patient sera by ELISA and Western blot. Conclusions. Antigenic proteins in the calcareous corpuscles of T. solium metacestodes deserve further analysis as candidates in the development of diagnostic tools for neurocysticercosis.
AB - Background. A protein fraction was isolated from calcareous corpuscles of Taenia solium cysticerci. The antigens in this fraction were recognized in ELISA and Western blot assays by all sera from a group of patients with active neurocysticercosis (NC) and were not recognized by the sera from patients with other neurological disorders. Western blot analysis also showed that several high molecular weight proteins were strongly recognized by antibodies in all the neurocysticercotic patient sera, suggesting a potential for serological diagnosis of neurocysticercosis. Methods. In order to characterize these antigenic proteins, we used a monoclonal antibody raised against a high MW calcium-binding protein associated with calcareous corpuscles of Echinococcus granulosus (EgCaBP1). Results. Western blot assays revealed the recognition of a protein band of about 260 kDa, appearing within the range of the high MW antigens recognized by the NC sera. Several cDNA clones were isolated through screening of a T. solium metacestode library with a DNA probe for EgCaBP1, containing partial coding sequences showing about 88% identity with the protein of E. granulosus. Moreover, a recombinant product expressed in bacteria from the partial coding sequence of T. solium showed the ability to bind Ca2+ and was recognized by the monoclonal antibody. This recombinant calcium-binding protein of T. solium was not recognized by the NC patient sera by ELISA and Western blot. Conclusions. Antigenic proteins in the calcareous corpuscles of T. solium metacestodes deserve further analysis as candidates in the development of diagnostic tools for neurocysticercosis.
KW - Ca
KW - Calcareous corpuscules
KW - Calcium-binding proteins
KW - Neurocysticercosis
KW - T. solium metacestode
UR - http://www.scopus.com/inward/record.url?scp=15244350315&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=15244350315&partnerID=8YFLogxK
U2 - 10.1016/j.arcmed.2004.10.001
DO - 10.1016/j.arcmed.2004.10.001
M3 - Article
C2 - 15777987
AN - SCOPUS:15244350315
SN - 0188-4409
VL - 36
SP - 4
EP - 9
JO - Archives of Medical Research
JF - Archives of Medical Research
IS - 1
ER -