Antioxidant vitamin C prevents decline in endothelial function during sitting

Saurabh S. Thosar, Sylvanna L. Bielko, Chad S. Wiggins, James E. Klaunig, Kieren J. Mather, Janet P. Wallace

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


Background: This study was designed to test the hypothesis that antioxidant Vitamin C prevents the impairment of endothelial function during prolonged sitting. Material/Methods: Eleven men (24.2±4.4 yrs) participated in 2 randomized 3-h sitting trials. In the sitting without vitamin C (SIT) and the sitting with vitamin C (VIT) trial, participants were seated for 3 h without moving their legs. Additionally, in the VIT trial, participants ingested 2 vitamin C tablets (1 g and 500 mg) at 30 min and 1 h 30 min, respectively. Superficial femoral artery (SFA) flow-mediated dilation (FMD) was measured hourly for 3 h. Results: By a 1-way ANOVA, there was a significant decline in FMD during 3 h of SIT (p<0.001). Simultaneously, there was a significant decline in antegrade (p=0.04) and mean (0.037) shear rates. For the SIT and VIT trials by a 2-way (trial x time) repeated measures ANOVA, there was a significant interaction (p=0.001). Pairwise testing revealed significant between-SFA FMD in the SIT and VIT trial at each hour after baseline, showing that VIT prevented the decline in FMD 1 h (p=0.009), 2 h (p=0.016), and 3 h (p=0.004). There was no difference in the shear rates between SIT and VIT trials (p>0.05). Conclusions: Three hours of sitting resulted in impaired SFA FMD. Antioxidant Vitamin C prevented the decline in SFA FMD, suggesting that oxidative stress may contribute to the impairment in endothelial function during sitting.

Original languageEnglish (US)
Pages (from-to)1015-1021
Number of pages7
JournalMedical Science Monitor
StatePublished - Apr 7 2015
Externally publishedYes


  • Antioxidants
  • Oxidative stress
  • Sedentary lifestyle

ASJC Scopus subject areas

  • Medicine(all)


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