TY - JOUR
T1 - Aquaporin-11 control of testicular fertility markers in Syrian hamsters
AU - Shannonhouse, John L.
AU - Urbanski, Henryk F.
AU - Woo, Shih Lung
AU - Fong, Li An
AU - Goddard, Scott D.
AU - Lucas, William F.
AU - Jones, Edward R.
AU - Wu, Chaodong
AU - Morgan, Caurnel
N1 - Funding Information:
This work was supported by a Texas AgriLife Research award (CM), a Heep Neuroscience Fellowship (JLS), and National Institutes of Health awards HD-024312 and AG-036670 (HFU).
PY - 2014/6/25
Y1 - 2014/6/25
N2 - The present study sought novel changes to the hamster testicular transcriptome during modulation of fertility by well-characterized photoperiodic stimuli. Transition from long days (LD, 14. h light/day) to short days (SD, 10. h light/day) triggered testicular regression (61% reduction of testis weight, relative to LD) in SD-sensitive (SD-S) hamsters within 16. weeks. After 22. weeks of SD exposure, a third cohort of hamsters became SD-refractory (SD-R), and exhibited testicular recrudescence (137% testis weight gain, relative to SD-S). Partial interrogation of the testicular transcriptome by annealing-control-primer-modified differential display PCR provided several candidates for regulation of testicular functions. Multiple linear regression modeling indicated the best correlation for aquaporin 11 ( Aqp11) with changes in testis weight. Correlations were also strongest for Aqp11 with expression levels of reference cDNAs that control spermatogenesis ( Hspa2 and Tnp2), steroidogenesis ( Cox2, 3βHsd, and Srebp2), sperm motility ( Catsper1, Pgk2, and Tnp2), inflammation ( Cox2), and apoptosis ( Bax and Bcl2). Moreover, siRNA-mediated knockdown of testicular Aqp11 mRNA and protein reduced Hspa2 and Tnp2 mRNA levels, and it increased 3βHsd mRNA levels. It also reduced mRNA levels for Sept12, which is a testis-specific inducer of spermatogenesis. These results suggest a central role for testicular Aqp11 signaling in the coordinate regulation of crucial components of fertility.
AB - The present study sought novel changes to the hamster testicular transcriptome during modulation of fertility by well-characterized photoperiodic stimuli. Transition from long days (LD, 14. h light/day) to short days (SD, 10. h light/day) triggered testicular regression (61% reduction of testis weight, relative to LD) in SD-sensitive (SD-S) hamsters within 16. weeks. After 22. weeks of SD exposure, a third cohort of hamsters became SD-refractory (SD-R), and exhibited testicular recrudescence (137% testis weight gain, relative to SD-S). Partial interrogation of the testicular transcriptome by annealing-control-primer-modified differential display PCR provided several candidates for regulation of testicular functions. Multiple linear regression modeling indicated the best correlation for aquaporin 11 ( Aqp11) with changes in testis weight. Correlations were also strongest for Aqp11 with expression levels of reference cDNAs that control spermatogenesis ( Hspa2 and Tnp2), steroidogenesis ( Cox2, 3βHsd, and Srebp2), sperm motility ( Catsper1, Pgk2, and Tnp2), inflammation ( Cox2), and apoptosis ( Bax and Bcl2). Moreover, siRNA-mediated knockdown of testicular Aqp11 mRNA and protein reduced Hspa2 and Tnp2 mRNA levels, and it increased 3βHsd mRNA levels. It also reduced mRNA levels for Sept12, which is a testis-specific inducer of spermatogenesis. These results suggest a central role for testicular Aqp11 signaling in the coordinate regulation of crucial components of fertility.
KW - Differential display PCR
KW - Mathematical modeling
KW - Photoperiod
KW - RNA interference
KW - Short interfering RNA
UR - http://www.scopus.com/inward/record.url?scp=84899929988&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84899929988&partnerID=8YFLogxK
U2 - 10.1016/j.mce.2014.04.011
DO - 10.1016/j.mce.2014.04.011
M3 - Article
C2 - 24791736
AN - SCOPUS:84899929988
SN - 0303-7207
VL - 391
SP - 1
EP - 9
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 1-2
ER -