Au-Loaded Superparamagnetic Mesoporous Bimetallic CoFeB Nanovehicles for Sensitive Autoantibody Detection

Yunqing Kang, Mostafa Kamal Masud, Yanna Guo, Yingji Zhao, Zakia Sultana Nishat, Jingjing Zhao, Bo Jiang, Yoshiyuki Sugahara, Tanja Pejovic, Terry Morgan, Md Shahriar A. Hossain, Hexing Li, Carlos Salomon, Toru Asahi, Yusuke Yamauchi

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Construction of a well-defined mesoporous nanostructure is crucial for applying nonnoble metals in catalysis and biomedicine owing to their highly exposed active sites and accessible surfaces. However, it remains a great challenge to controllably synthesize superparamagnetic CoFe-based mesoporous nanospheres with tunable compositions and exposed large pores, which are sought for immobilization or adsorption of guest molecules for magnetic capture, isolation, preconcentration, and purification. Herein, a facile assembly strategy of a block copolymer was developed to fabricate a mesoporous CoFeB amorphous alloy with abundant metallic Co/Fe atoms, which served as an ideal scaffold for well-dispersed loading of Au nanoparticles (∼3.1 nm) via the galvanic replacement reaction. The prepared Au-CoFeB possessed high saturation magnetization as well as uniform and large open mesopores (∼12.5 nm), which provided ample accessibility to biomolecules, such as nucleic acids, enzymes, proteins, and antibodies. Through this distinctive combination of superparamagnetism (CoFeB) and biofavorability (Au), the resulting Au-CoFeB was employed as a dispersible nanovehicle for the direct capture and isolation of p53 autoantibody from serum samples. Highly sensitive detection of the autoantibody was achieved with a limit of detection of 0.006 U/mL, which was 50 times lower than that of the conventional p53-ELISA kit-based detection system. Our assay is capable of quantifying differential expression patterns for detecting p53 autoantibodies in ovarian cancer patients. This assay provides a rapid, inexpensive, and portable platform with the potential to detect a wide range of clinically relevant protein biomarkers.

Original languageEnglish (US)
Pages (from-to)3346-3357
Number of pages12
JournalACS Nano
Volume17
Issue number4
DOIs
StatePublished - Feb 28 2023

Keywords

  • gold nanoparticles
  • magnetic bimetallic alloys
  • mesoporous nonnoble metals
  • p53 autoantibody
  • superparamagnetism

ASJC Scopus subject areas

  • General Materials Science
  • General Engineering
  • General Physics and Astronomy

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