In this Article, we highlighted important residues that could affect Piezo1 gating. Accordingly, in Fig. 3 and Extended Data Fig. 7 of the Article, we measured mechanically activated currents from cells that express alanine substitution mutants (M2493A and F2494A) of residues that we predicted as the hydrophobic gate of the channel. Our experiments showed that the single mutants cause a gain-of-function phenotype (Fig. 3g–i). As a control, we also tested the double mutant M2493A/F2494A, which we found to be non-functional (Extended Data Fig. 7a,b).
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