TY - JOUR
T1 - Automated counting of T cell rosettes
AU - Vandenbark, Arthur A.
AU - Mark Vetto, R.
N1 - Funding Information:
* This research was supported by project number 8958-01 from the Veterans Administration Hospital, Portland, Oregon 97207.
PY - 1976/3
Y1 - 1976/3
N2 - Human T cell rosettes were enumerated using an automated particle counter, the Bio/Physics Cytograf 6300A. An electronic oscilloscope representation of particle absorbance and scatter of a focused laser beam allows the separation and enumeration of both rosetted and non-rosetted lymphocytes. Repeated cytograf sampling of a single rosette preparation gave highly reproducible results, and sampling from replicate tubes produced the same degree of variation as microscopic analysis. T cell rosettes prepared from 27 volunteers and compared by both methods of quantitation showed a high degree of correlation. This method can objectively measure at least 100 times as many cells for their rosette-forming capability as the tedious microscopic technique.
AB - Human T cell rosettes were enumerated using an automated particle counter, the Bio/Physics Cytograf 6300A. An electronic oscilloscope representation of particle absorbance and scatter of a focused laser beam allows the separation and enumeration of both rosetted and non-rosetted lymphocytes. Repeated cytograf sampling of a single rosette preparation gave highly reproducible results, and sampling from replicate tubes produced the same degree of variation as microscopic analysis. T cell rosettes prepared from 27 volunteers and compared by both methods of quantitation showed a high degree of correlation. This method can objectively measure at least 100 times as many cells for their rosette-forming capability as the tedious microscopic technique.
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U2 - 10.1016/0022-1759(76)90176-9
DO - 10.1016/0022-1759(76)90176-9
M3 - Article
C2 - 1084373
AN - SCOPUS:0017236542
SN - 0022-1759
VL - 10
SP - 261
EP - 270
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 2-3
ER -