TY - JOUR
T1 - Automated spectroscopic retinal oximetry with visible-light optical coherence tomography
AU - Pi, Shaohua
AU - Camino, Acner
AU - Cepurna, William
AU - Wei, Xiang
AU - Zhang, Miao
AU - Huang, David
AU - Morrison, John
AU - Jia, Yali
N1 - Funding Information:
National Institutes of Health (R01 EY027833, DP3 DK104397, R01 EY024544, R01 EY023285, R01 EY010145, P30 EY010572); unrestricted departmental funding grant and William & Mary Greve Special Scholar Award from Research to Prevent Blindness (New York, NY).
Publisher Copyright:
© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
PY - 2018/5/1
Y1 - 2018/5/1
N2 - Accurate, quantitative assessment of retinal blood oxygen saturation (sO2) may provide a useful early indicator of pathophysiology in several ocular diseases. Here, with visible-light optical coherence tomography (OCT), we demonstrate an automated spectroscopic retinal oximetry algorithm to measure the sO2 within the retinal arteries (A-sO2) and veins (V-sO2) in rats by automatically detecting the vascular posterior boundary on crosssectional structural OCT. The algorithm was validated in vitro with flow phantoms and in vivo in rats by comparing the sO2 results, respectively, to those obtained using a blood gas analyzer and pulse oximetry. We also investigated the response of oxygen extraction (A-V sO2), including inter-session reproducibility, at different inhaled oxygen concentrations.
AB - Accurate, quantitative assessment of retinal blood oxygen saturation (sO2) may provide a useful early indicator of pathophysiology in several ocular diseases. Here, with visible-light optical coherence tomography (OCT), we demonstrate an automated spectroscopic retinal oximetry algorithm to measure the sO2 within the retinal arteries (A-sO2) and veins (V-sO2) in rats by automatically detecting the vascular posterior boundary on crosssectional structural OCT. The algorithm was validated in vitro with flow phantoms and in vivo in rats by comparing the sO2 results, respectively, to those obtained using a blood gas analyzer and pulse oximetry. We also investigated the response of oxygen extraction (A-V sO2), including inter-session reproducibility, at different inhaled oxygen concentrations.
KW - Functional monitoring and imaging
KW - Image enhancement
KW - Ophthalmology
KW - Optical coherence tomography
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U2 - 10.1364/BOE.9.002056
DO - 10.1364/BOE.9.002056
M3 - Article
AN - SCOPUS:85046342055
SN - 2156-7085
VL - 9
SP - 2056
EP - 2067
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 5
M1 - #320440
ER -