Best practices for managing large CryoEM facilities

Bart Alewijnse; Alun W. Ashton; Melissa G. Chambers; Songye Chen; Anchi Cheng; Mark Ebrahim; Edward T. Eng; Wim J.H. Hagen; Abraham J. Koster; Claudia S. López; Natalya Lukoyanova; Joaquin Ortega; Ludovic Renault; Steve Reyntjens; William J. Rice; Giovanna Scapin; Raymond Schrijver; Alistair Siebert; Scott M. Stagg; Valerie Grum-Tokars; Elizabeth R. Wright; Shenping Wu; Zhiheng Yu; Z. Hong Zhou; Bridget Carragher; Clinton S. Potter

doi:10.1016/j.jsb.2017.07.011

Best practices for managing large CryoEM facilities

Bart Alewijnse, Alun W. Ashton, Melissa G. Chambers, Songye Chen, Anchi Cheng, Mark Ebrahim, Edward T. Eng, Wim J.H. Hagen, Abraham J. Koster, Claudia S. López, Natalya Lukoyanova, Joaquin Ortega, Ludovic Renault, Steve Reyntjens, William J. Rice, Giovanna Scapin, Raymond Schrijver, Alistair Siebert, Scott M. Stagg, Valerie Grum-TokarsElizabeth R. Wright, Shenping Wu, Zhiheng Yu, Z. Hong Zhou, Bridget Carragher, Clinton S. Potter

Research output: Contribution to journal › Review article › peer-review

26 Scopus citations

Abstract

This paper provides an overview of the discussion and presentations from the Workshop on the Management of Large CryoEM Facilities held at the New York Structural Biology Center, New York, NY on February 6–7, 2017. A major objective of the workshop was to discuss best practices for managing cryoEM facilities. The discussions were largely focused on supporting single-particle methods for cryoEM and topics included: user access, assessing projects, workflow, sample handling, microscopy, data management and processing, and user training.

Original language	English (US)
Pages (from-to)	225-236
Number of pages	12
Journal	Journal of Structural Biology
Volume	199
Issue number	3
DOIs	https://doi.org/10.1016/j.jsb.2017.07.011
State	Published - Sep 2017

Keywords

Automation
Cryotem
Data management
Facility management
Single-particle
User training

ASJC Scopus subject areas

Structural Biology

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10.1016/j.jsb.2017.07.011

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Alewijnse, B., Ashton, A. W., Chambers, M. G., Chen, S., Cheng, A., Ebrahim, M., Eng, E. T., Hagen, W. J. H., Koster, A. J., López, C. S., Lukoyanova, N., Ortega, J., Renault, L., Reyntjens, S., Rice, W. J., Scapin, G., Schrijver, R., Siebert, A., Stagg, S. M., ... Potter, C. S. (2017). Best practices for managing large CryoEM facilities. Journal of Structural Biology, 199(3), 225-236. https://doi.org/10.1016/j.jsb.2017.07.011

Alewijnse, B, Ashton, AW, Chambers, MG, Chen, S, Cheng, A, Ebrahim, M, Eng, ET, Hagen, WJH, Koster, AJ, López, CS, Lukoyanova, N, Ortega, J, Renault, L, Reyntjens, S, Rice, WJ, Scapin, G, Schrijver, R, Siebert, A, Stagg, SM, Grum-Tokars, V, Wright, ER, Wu, S, Yu, Z, Zhou, ZH, Carragher, B & Potter, CS 2017, 'Best practices for managing large CryoEM facilities', Journal of Structural Biology, vol. 199, no. 3, pp. 225-236. https://doi.org/10.1016/j.jsb.2017.07.011

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title = "Best practices for managing large CryoEM facilities",

abstract = "This paper provides an overview of the discussion and presentations from the Workshop on the Management of Large CryoEM Facilities held at the New York Structural Biology Center, New York, NY on February 6–7, 2017. A major objective of the workshop was to discuss best practices for managing cryoEM facilities. The discussions were largely focused on supporting single-particle methods for cryoEM and topics included: user access, assessing projects, workflow, sample handling, microscopy, data management and processing, and user training.",

keywords = "Automation, Cryotem, Data management, Facility management, Single-particle, User training",

author = "Bart Alewijnse and Ashton, {Alun W.} and Chambers, {Melissa G.} and Songye Chen and Anchi Cheng and Mark Ebrahim and Eng, {Edward T.} and Hagen, {Wim J.H.} and Koster, {Abraham J.} and L{\'o}pez, {Claudia S.} and Natalya Lukoyanova and Joaquin Ortega and Ludovic Renault and Steve Reyntjens and Rice, {William J.} and Giovanna Scapin and Raymond Schrijver and Alistair Siebert and Stagg, {Scott M.} and Valerie Grum-Tokars and Wright, {Elizabeth R.} and Shenping Wu and Zhiheng Yu and Zhou, {Z. Hong} and Bridget Carragher and Potter, {Clinton S.}",

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doi = "10.1016/j.jsb.2017.07.011",

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AU - Cheng, Anchi

AU - Ebrahim, Mark

AU - Eng, Edward T.

AU - Hagen, Wim J.H.

AU - Koster, Abraham J.

AU - López, Claudia S.

AU - Lukoyanova, Natalya

AU - Ortega, Joaquin

AU - Renault, Ludovic

AU - Reyntjens, Steve

AU - Rice, William J.

AU - Scapin, Giovanna

AU - Schrijver, Raymond

AU - Siebert, Alistair

AU - Stagg, Scott M.

AU - Grum-Tokars, Valerie

AU - Wright, Elizabeth R.

AU - Wu, Shenping

AU - Yu, Zhiheng

AU - Zhou, Z. Hong

AU - Carragher, Bridget

AU - Potter, Clinton S.

PY - 2017/9

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KW - Automation

KW - Cryotem

KW - Data management

KW - Facility management

KW - Single-particle

KW - User training

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JF - Journal of Structural Biology

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Best practices for managing large CryoEM facilities

Abstract

Keywords

ASJC Scopus subject areas

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