BH3-only protein Bim inhibits activity of antiapoptotic members of Bcl-2 family when expressed in yeast

Barbora Juhásová; Marek Mentel; Ingrid Bhatia-Kiššová; Igor Zeman; Jordan Kolarov; Michael Forte; Peter Polčic

doi:10.1016/j.febslet.2011.07.027

BH3-only protein Bim inhibits activity of antiapoptotic members of Bcl-2 family when expressed in yeast

Barbora Juhásová, Marek Mentel, Ingrid Bhatia-Kiššová, Igor Zeman, Jordan Kolarov, Michael Forte, Peter Polčic

Vollum Institute

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Proteins of the Bcl-2 family regulate programmed cell death in mammals by promoting the release of cytochrome c from mitochondria in response to various proapoptotic stimuli. The mechanism by which BH3-only members of the family activate multidomain proapoptotic proteins Bax and Bak to form a pore in mitochondrial membranes remains under dispute. We report that cell death promoting activity of BH3-only protein Bim can be reconstituted in yeast when both Bax and antiapoptotic protein Bcl-X_L are present, suggesting that Bim likely activates Bax indirectly by inhibiting antiapoptotic proteins.

Original language	English (US)
Pages (from-to)	2709-2713
Number of pages	5
Journal	FEBS Letters
Volume	585
Issue number	17
DOIs	https://doi.org/10.1016/j.febslet.2011.07.027
State	Published - Sep 2 2011

Keywords

Apoptosis
BH3-only proteins
Bax
Bcl-X
Bim
Yeast

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2011.07.027

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title = "BH3-only protein Bim inhibits activity of antiapoptotic members of Bcl-2 family when expressed in yeast",

abstract = "Proteins of the Bcl-2 family regulate programmed cell death in mammals by promoting the release of cytochrome c from mitochondria in response to various proapoptotic stimuli. The mechanism by which BH3-only members of the family activate multidomain proapoptotic proteins Bax and Bak to form a pore in mitochondrial membranes remains under dispute. We report that cell death promoting activity of BH3-only protein Bim can be reconstituted in yeast when both Bax and antiapoptotic protein Bcl-XL are present, suggesting that Bim likely activates Bax indirectly by inhibiting antiapoptotic proteins.",

keywords = "Apoptosis, BH3-only proteins, Bax, Bcl-X, Bim, Yeast",

author = "Barbora Juh{\'a}sov{\'a} and Marek Mentel and Ingrid Bhatia-Ki{\v s}{\v s}ov{\'a} and Igor Zeman and Jordan Kolarov and Michael Forte and Peter Pol{\v c}ic",

note = "Funding Information: This work was supported by grants from Slovak grant agency VEGA No. 1/0870/11 and by Science and Technology Assistance Agency No. APVV-0024-07. ",

year = "2011",

month = sep,

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doi = "10.1016/j.febslet.2011.07.027",

language = "English (US)",

volume = "585",

pages = "2709--2713",

journal = "FEBS Letters",

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TY - JOUR

T1 - BH3-only protein Bim inhibits activity of antiapoptotic members of Bcl-2 family when expressed in yeast

AU - Juhásová, Barbora

AU - Mentel, Marek

AU - Bhatia-Kiššová, Ingrid

AU - Zeman, Igor

AU - Kolarov, Jordan

AU - Forte, Michael

AU - Polčic, Peter

N1 - Funding Information: This work was supported by grants from Slovak grant agency VEGA No. 1/0870/11 and by Science and Technology Assistance Agency No. APVV-0024-07.

PY - 2011/9/2

Y1 - 2011/9/2

N2 - Proteins of the Bcl-2 family regulate programmed cell death in mammals by promoting the release of cytochrome c from mitochondria in response to various proapoptotic stimuli. The mechanism by which BH3-only members of the family activate multidomain proapoptotic proteins Bax and Bak to form a pore in mitochondrial membranes remains under dispute. We report that cell death promoting activity of BH3-only protein Bim can be reconstituted in yeast when both Bax and antiapoptotic protein Bcl-XL are present, suggesting that Bim likely activates Bax indirectly by inhibiting antiapoptotic proteins.

AB - Proteins of the Bcl-2 family regulate programmed cell death in mammals by promoting the release of cytochrome c from mitochondria in response to various proapoptotic stimuli. The mechanism by which BH3-only members of the family activate multidomain proapoptotic proteins Bax and Bak to form a pore in mitochondrial membranes remains under dispute. We report that cell death promoting activity of BH3-only protein Bim can be reconstituted in yeast when both Bax and antiapoptotic protein Bcl-XL are present, suggesting that Bim likely activates Bax indirectly by inhibiting antiapoptotic proteins.

KW - Apoptosis

KW - BH3-only proteins

KW - Bax

KW - Bcl-X

KW - Bim

KW - Yeast

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DO - 10.1016/j.febslet.2011.07.027

M3 - Article

C2 - 21820438

AN - SCOPUS:81155132521

SN - 0014-5793

VL - 585

SP - 2709

EP - 2713

JO - FEBS Letters

JF - FEBS Letters

IS - 17

ER -

BH3-only protein Bim inhibits activity of antiapoptotic members of Bcl-2 family when expressed in yeast

Abstract

Keywords

ASJC Scopus subject areas

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