Abstract
Background: We developed an aligned bi-layered vascular graft derived from human induced pluripotent stem cells (iPSCs) that recapitulates the cellular composition, orientation, and anti-inflammatory function of blood vessels. Materials & methods: The luminal layer consisted of longitudinal-aligned nanofibrillar collagen containing primary endothelial cells (ECs) or iPSC-derived ECs (iPSC-ECs). The outer layer contained circumferentially oriented nanofibrillar collagen with primary smooth muscle cells (SMCs) or iPSC-derived SMCs(iPSC-SMCs). Results: On the aligned scaffolds, cells organized F-actin assembly within 8° from the direction of nanofibrils. When compared to randomly-oriented scaffolds, EC-seeded aligned scaffolds had significant reduced inflammatory response, based on adhesivity to monocytes. Conclusion: This study highlights the importance of anisotropic scaffolds in directing cell form and function, and has therapeutic significance as physiologically relevant blood vessels.
Original language | English (US) |
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Pages (from-to) | 745-755 |
Number of pages | 11 |
Journal | Regenerative Medicine |
Volume | 10 |
Issue number | 6 |
DOIs | |
State | Published - Sep 2015 |
Externally published | Yes |
Keywords
- collagen
- endothelial
- extracellular matrix
- iPSC
- nanofibrillar
- scaffolds
- smooth muscle
- stem cells
- tissue engineering
- vascular graft
ASJC Scopus subject areas
- Biomedical Engineering
- Embryology