Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress

C. S. López; H. Heras; H. Garda; S. Ruzal; C. Sánchez-Rivas; E. Rivas

doi:10.1016/S0168-1605(00)00171-9

Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress

C. S. López, H. Heras, H. Garda, S. Ruzal, C. Sánchez-Rivas, E. Rivas

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

The behaviour and state of the envelopes from B. subtilis cultures grown in Luria Bertani (LB) medium with and without 1.5 M NaCl are compared. Under hypertonic conditions, the hydrophobicity of the cultures increases. The phospholipid and fatty acid (FA) compositions show important differences: a higher cardiolipin (CL) content [at the expense of phosphatidylglycerol (PG)], and a higher unsaturated and straight chain FA content. The fluidity of the membranes, determined with fluorescent probes, indicates an increase in viscosity of the cytoplasmic membrane. The consequences of these variations in membrane permeability and osmotolerance are discussed. Copyright (C) 2000 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	137-142
Number of pages	6
Journal	International Journal of Food Microbiology
Volume	55
Issue number	1-3
DOIs	https://doi.org/10.1016/S0168-1605(00)00171-9
State	Published - Apr 10 2000
Externally published	Yes

Keywords

Bacillus subtilis
Fatty acids
Membranes
Phospholipids
Stress

ASJC Scopus subject areas

Food Science
Microbiology

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10.1016/S0168-1605(00)00171-9

Cite this

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title = "Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress",

abstract = "The behaviour and state of the envelopes from B. subtilis cultures grown in Luria Bertani (LB) medium with and without 1.5 M NaCl are compared. Under hypertonic conditions, the hydrophobicity of the cultures increases. The phospholipid and fatty acid (FA) compositions show important differences: a higher cardiolipin (CL) content [at the expense of phosphatidylglycerol (PG)], and a higher unsaturated and straight chain FA content. The fluidity of the membranes, determined with fluorescent probes, indicates an increase in viscosity of the cytoplasmic membrane. The consequences of these variations in membrane permeability and osmotolerance are discussed. Copyright (C) 2000 Elsevier Science B.V.",

keywords = "Bacillus subtilis, Fatty acids, Membranes, Phospholipids, Stress",

author = "L{\'o}pez, {C. S.} and H. Heras and H. Garda and S. Ruzal and C. S{\'a}nchez-Rivas and E. Rivas",

note = "Funding Information: This work was carried out with the aid of grants from CONICET (Consejo Nacional de Investigaciones Cient{\'ı}ficas y T{\'e}cnicas), ANPCYT (Agencia Nacional de Promoci{\'o}n Cient{\'ı}fica y Tecnol{\'o}gica) and the University of Buenos Aires (Argentina)",

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doi = "10.1016/S0168-1605(00)00171-9",

language = "English (US)",

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T1 - Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress

AU - López, C. S.

AU - Heras, H.

AU - Garda, H.

AU - Ruzal, S.

AU - Sánchez-Rivas, C.

AU - Rivas, E.

N1 - Funding Information: This work was carried out with the aid of grants from CONICET (Consejo Nacional de Investigaciones Cientı́ficas y Técnicas), ANPCYT (Agencia Nacional de Promoción Cientı́fica y Tecnológica) and the University of Buenos Aires (Argentina)

PY - 2000/4/10

Y1 - 2000/4/10

N2 - The behaviour and state of the envelopes from B. subtilis cultures grown in Luria Bertani (LB) medium with and without 1.5 M NaCl are compared. Under hypertonic conditions, the hydrophobicity of the cultures increases. The phospholipid and fatty acid (FA) compositions show important differences: a higher cardiolipin (CL) content [at the expense of phosphatidylglycerol (PG)], and a higher unsaturated and straight chain FA content. The fluidity of the membranes, determined with fluorescent probes, indicates an increase in viscosity of the cytoplasmic membrane. The consequences of these variations in membrane permeability and osmotolerance are discussed. Copyright (C) 2000 Elsevier Science B.V.

AB - The behaviour and state of the envelopes from B. subtilis cultures grown in Luria Bertani (LB) medium with and without 1.5 M NaCl are compared. Under hypertonic conditions, the hydrophobicity of the cultures increases. The phospholipid and fatty acid (FA) compositions show important differences: a higher cardiolipin (CL) content [at the expense of phosphatidylglycerol (PG)], and a higher unsaturated and straight chain FA content. The fluidity of the membranes, determined with fluorescent probes, indicates an increase in viscosity of the cytoplasmic membrane. The consequences of these variations in membrane permeability and osmotolerance are discussed. Copyright (C) 2000 Elsevier Science B.V.

KW - Bacillus subtilis

KW - Fatty acids

KW - Membranes

KW - Phospholipids

KW - Stress

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AN - SCOPUS:0342859367

SN - 0168-1605

VL - 55

SP - 137

EP - 142

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

IS - 1-3

ER -

Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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