TY - JOUR
T1 - Biofilm Formation of Oral and Endodontic Enterococcus faecalis
AU - Duggan, Jason M.
AU - Sedgley, Christine M.
N1 - Funding Information:
Supported by the Endodontic Research Fund at The University of Michigan.
PY - 2007/7
Y1 - 2007/7
N2 - Biofilms are complex aggregations of microorganisms attached to a surface. The formation of biofilms might facilitate certain survival and virulence characteristics under some situations. This study tested the hypothesis that the ability of Enterococcus faecalis to form biofilms is related to the source of the strains. E. faecalis strains recovered from root canals (n = 33), the oral cavity (n = 21), and non-oral/non-endodontic sources (n = 16) were studied. Biofilms were grown in tryptic soy broth in 96-well plates for 24 hours at 37°C, fixed with Bouin's fixative, and stained with 1% crystal violet. Optical density at 570 nm (OD570) was measured by using a microtiter plate reader. Experiments were performed in quadruplicate on three occasions and mean OD570 readings determined for each strain. There were no statistically significant differences between groups (p = 0.066, Kruskal-Wallis). Within the root canal and oral isolates there were no significant associations between biofilm formation and the presence of the virulence determinants asa, cylA, esp, and gelE.
AB - Biofilms are complex aggregations of microorganisms attached to a surface. The formation of biofilms might facilitate certain survival and virulence characteristics under some situations. This study tested the hypothesis that the ability of Enterococcus faecalis to form biofilms is related to the source of the strains. E. faecalis strains recovered from root canals (n = 33), the oral cavity (n = 21), and non-oral/non-endodontic sources (n = 16) were studied. Biofilms were grown in tryptic soy broth in 96-well plates for 24 hours at 37°C, fixed with Bouin's fixative, and stained with 1% crystal violet. Optical density at 570 nm (OD570) was measured by using a microtiter plate reader. Experiments were performed in quadruplicate on three occasions and mean OD570 readings determined for each strain. There were no statistically significant differences between groups (p = 0.066, Kruskal-Wallis). Within the root canal and oral isolates there were no significant associations between biofilm formation and the presence of the virulence determinants asa, cylA, esp, and gelE.
KW - Biofilm
KW - Enterococcus faecalis
KW - endodontic
KW - in vitro
KW - oral
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U2 - 10.1016/j.joen.2007.02.016
DO - 10.1016/j.joen.2007.02.016
M3 - Article
C2 - 17804318
AN - SCOPUS:34250162489
SN - 0099-2399
VL - 33
SP - 815
EP - 818
JO - Journal of endodontics
JF - Journal of endodontics
IS - 7
ER -