Bleomycin-treated DNA is specifically cleaved only by endonuclease IV in E. coli

Michael E. Hagensee; Robb E. Moses

doi:10.1016/0167-4781(90)90016-U

Bleomycin-treated DNA is specifically cleaved only by endonuclease IV in E. coli

Michael E. Hagensee, Robb E. Moses

Molecular and Medical Genetics

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

We have isolated an endonuclease from E. coli active on bleomycin-treated DNA. Purification on DEAE-cellulose separated this activity in strains lacking endonuclease I, endonuclease III or exonuclease III. After DEAE chromatography, the enzyme was active in the absence of divalent cations and was not inhibited by tRNA or harmane. In addition, this enzyme was stable at 45°C for 20 min. These properties are consistent with this activity being endonuclease IV. This was supported by our finding no activity in a strain lacking endonuclease IV.

Original language	English (US)
Pages (from-to)	19-23
Number of pages	5
Journal	BBA - Gene Structure and Expression
Volume	1048
Issue number	1
DOIs	https://doi.org/10.1016/0167-4781(90)90016-U
State	Published - Jan 30 1990

Keywords

(E. coli)
AP endonuclease
Bleomycin
Endonuclease IV

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/0167-4781(90)90016-U

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@article{4cbd2fac8e45432888e5197284b8d6fa,

title = "Bleomycin-treated DNA is specifically cleaved only by endonuclease IV in E. coli",

abstract = "We have isolated an endonuclease from E. coli active on bleomycin-treated DNA. Purification on DEAE-cellulose separated this activity in strains lacking endonuclease I, endonuclease III or exonuclease III. After DEAE chromatography, the enzyme was active in the absence of divalent cations and was not inhibited by tRNA or harmane. In addition, this enzyme was stable at 45°C for 20 min. These properties are consistent with this activity being endonuclease IV. This was supported by our finding no activity in a strain lacking endonuclease IV.",

keywords = "(E. coli), AP endonuclease, Bleomycin, Endonuclease IV",

author = "Hagensee, {Michael E.} and Moses, {Robb E.}",

note = "Funding Information: We have isolated an endonuclease from E. coli active on bleomycin-treated DNA. Purification on DEAE-cellulose separated this activity in strains lacking endonuclease I, endonuclease III or exonuclease III. After DEAE chromatography, the enzyme was active in the absence of divalent cations and was not inhibited by tRNA or harmane. In addition, this enzyme was stable at 45 °C for 20 min. These properties are consistent with this activity being endonuclease IV. This was supported by our finding no activity in a strain lacking endonuclease IV.",

year = "1990",

month = jan,

day = "30",

doi = "10.1016/0167-4781(90)90016-U",

language = "English (US)",

volume = "1048",

pages = "19--23",

journal = "BBA - Gene Structure and Expression",

issn = "0167-4781",

publisher = "Elsevier BV",

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TY - JOUR

T1 - Bleomycin-treated DNA is specifically cleaved only by endonuclease IV in E. coli

AU - Hagensee, Michael E.

AU - Moses, Robb E.

N1 - Funding Information: We have isolated an endonuclease from E. coli active on bleomycin-treated DNA. Purification on DEAE-cellulose separated this activity in strains lacking endonuclease I, endonuclease III or exonuclease III. After DEAE chromatography, the enzyme was active in the absence of divalent cations and was not inhibited by tRNA or harmane. In addition, this enzyme was stable at 45 °C for 20 min. These properties are consistent with this activity being endonuclease IV. This was supported by our finding no activity in a strain lacking endonuclease IV.

PY - 1990/1/30

Y1 - 1990/1/30

N2 - We have isolated an endonuclease from E. coli active on bleomycin-treated DNA. Purification on DEAE-cellulose separated this activity in strains lacking endonuclease I, endonuclease III or exonuclease III. After DEAE chromatography, the enzyme was active in the absence of divalent cations and was not inhibited by tRNA or harmane. In addition, this enzyme was stable at 45°C for 20 min. These properties are consistent with this activity being endonuclease IV. This was supported by our finding no activity in a strain lacking endonuclease IV.

AB - We have isolated an endonuclease from E. coli active on bleomycin-treated DNA. Purification on DEAE-cellulose separated this activity in strains lacking endonuclease I, endonuclease III or exonuclease III. After DEAE chromatography, the enzyme was active in the absence of divalent cations and was not inhibited by tRNA or harmane. In addition, this enzyme was stable at 45°C for 20 min. These properties are consistent with this activity being endonuclease IV. This was supported by our finding no activity in a strain lacking endonuclease IV.

KW - (E. coli)

KW - AP endonuclease

KW - Bleomycin

KW - Endonuclease IV

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UR - http://www.scopus.com/inward/citedby.url?scp=0025022615&partnerID=8YFLogxK

U2 - 10.1016/0167-4781(90)90016-U

DO - 10.1016/0167-4781(90)90016-U

M3 - Article

C2 - 1688711

AN - SCOPUS:0025022615

SN - 0167-4781

VL - 1048

SP - 19

EP - 23

JO - BBA - Gene Structure and Expression

JF - BBA - Gene Structure and Expression

IS - 1

ER -

Bleomycin-treated DNA is specifically cleaved only by endonuclease IV in E. coli

Abstract

Keywords

ASJC Scopus subject areas

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