Abstract
Cyclic adenosine monophosphate (cAMP) has tissue-specific effects on growth, differentiation, and gene expression. We show here that cAMP can activate the transcription factor Elk-1 and induce neuronal differentiation of PC12 cells via its activation of the MAP kinase cascade. These cell type- specific actions of cAMP require the expression of the serine/threonine kinase B-Raf and activation of the small G protein Rap1. Rap1, activated by mutation or by the cAMP-dependent protein kinase PKA, is a selective activator of B-Raf and an inhibitor of Raf-1. Therefore, in B-Raf-expressing cells, the activation of Rap1 provides a mechanism for tissue-specific regulation of cell growth and differentiation via MAP kinase.
Original language | English (US) |
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Pages (from-to) | 73-82 |
Number of pages | 10 |
Journal | Cell |
Volume | 89 |
Issue number | 1 |
DOIs | |
State | Published - Apr 4 1997 |
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology