Cardiac Light-Sheet Fluorescent Microscopy for Multi-Scale and Rapid Imaging of Architecture and Function

Peng Fei, Juhyun Lee, René R.Sevag Packard, Konstantina Ioanna Sereti, Hao Xu, Jianguo Ma, Yichen Ding, Hanul Kang, Harrison Chen, Kevin Sung, Rajan Kulkarni, Reza Ardehali, C. C.Jay Kuo, Xiaolei Xu, Chih Ming Ho, Tzung K. Hsiai

Research output: Contribution to journalArticlepeer-review

52 Scopus citations


Light Sheet Fluorescence Microscopy (LSFM) enables multi-dimensional and multi-scale imaging via illuminating specimens with a separate thin sheet of laser. It allows rapid plane illumination for reduced photo-damage and superior axial resolution and contrast. We hereby demonstrate cardiac LSFM (c-LSFM) imaging to assess the functional architecture of zebrafish embryos with a retrospective cardiac synchronization algorithm for four-dimensional reconstruction (3-D space + time). By combining our approach with tissue clearing techniques, we reveal the entire cardiac structures and hypertrabeculation of adult zebrafish hearts in response to doxorubicin treatment. By integrating the resolution enhancement technique with c-LSFM to increase the resolving power under a large field-of-view, we demonstrate the use of low power objective to resolve the entire architecture of large-scale neonatal mouse hearts, revealing the helical orientation of individual myocardial fibers. Therefore, our c-LSFM imaging approach provides multi-scale visualization of architecture and function to drive cardiovascular research with translational implication in congenital heart diseases.

Original languageEnglish (US)
Article number22489
JournalScientific Reports
StatePublished - Mar 3 2016
Externally publishedYes

ASJC Scopus subject areas

  • General


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