Categorial selection of the antibody repertoire in splenic B cells

Robert L. Schelonka, Jason Tanner, Yingxin Zhuang, G. Larry Gartland, Michael Zemlin, Harry W. Schroeder

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


In the bone marrow, the passage of developing B ceAs-through critical checkpoints of differentiation is associated with a reduction of specific categories of CDR3 of the Ig heavy chain (CDR-H3), particularly those with excessive hydrophobic or charged amino acids and those with a length of eight or fewer residues. To gain insight into the role of CDR-H3 content in the development of B cells in the spleen, we compared the sequences of VH7183DJCμ transcripts from sorted transitional T1, marginal zone, and follicular B cell subsets to those expressed by immature IgM+IgD- and mature lgMloIgDhi B cells in the bone marrow. Although differences in VH utilization were noted, the T1 CDR-H3 repertoire showed extensive similarity to that of immature bone marrow B cells, and the follicular CDR-H3 repertoire most resembled that of mature bone marrow B cells. Unlike the splenic follicular and bone marrow mature B cell CDR-H3 repertoires, the marginal zone B cell CDR-H3 repertoire retained both short and highly charged amino acid motifs, including those with two arginines. Our findings suggest that antigen binding sites containing specific categories of CDR-H3 sequence content may inhibit, permit, or even facilitate passage of the host B cell through critical checkpoints in peripheral as well as central development.

Original languageEnglish (US)
Pages (from-to)1010-1021
Number of pages12
JournalEuropean Journal of Immunology
Issue number4
StatePublished - Apr 2007
Externally publishedYes


  • Antibodies
  • B cells
  • Repertoire development
  • Rodent
  • Spleen

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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