Cellular serine/threonine phosphatase activity during human cytomegalovirus infection

Morgan Hakki, Adam P. Geballe

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


While the importance of cellular and viral kinases in HCMV replication has been demonstrated, relatively little is known about the activity of cellular phosphatases. We conducted a series of experiments designed to investigate the effect of HCMV infection on cellular serine/threonine phosphatase activity. We found that the abundance of two major cellular serine/threonine phosphatases, PP1 and PP2A, increases during HCMV infection. This was associated with an increase in threonine phosphatase activity in HCMV-infected cells. HCMV infection conferred resistance to the effects of the phosphatase inhibitors calyculin A (CA) and okadaic acid with regards to global protein hyperphosphorylation and the shutoff of protein synthesis. The protective effect of HCMV infection could be overcome at a high concentration of CA, suggesting that cellular phosphatase activity is required for critical cellular processes during HCMV infection. Specifically, phosphatase activity was required to limit the accumulation of phospho-eIF2α, but not phospho-PKR, during HCMV infection.

Original languageEnglish (US)
Pages (from-to)255-263
Number of pages9
Issue number2
StatePublished - Oct 25 2008
Externally publishedYes


  • Cytomegalovirus
  • Phosphatase

ASJC Scopus subject areas

  • Virology


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