Cep72 regulates the localization of key centrosomal proteins and proper bipolar spindle formation

Naoki Oshimori, Xue Li, Miho Ohsugi, Tadashi Yamamoto

Research output: Contribution to journalArticlepeer-review

65 Scopus citations


Microtubule-nucleation activity and structural integrity of the centrosome are critical for various cellular functions. The γ-tubulin ring complexes (γTuRCs) localizing to the pericentriolar matrix (PCM) of the centrosome are major sites of microtubule nucleation. The PCM is thought to be created by two cognate large coiled-coil proteins, pericentrin/kendrin and CG-NAP/AKAP450, and its stabilization by Kizuna is essential for bipolar spindle formation. However, the mechanisms by which these proteins are recruited and organized into a proper structure with microtubule-organizing activity are poorly understood. Here we identify a centrosomal protein Cep72 as a Kizuna-interacting protein. Interestingly, Cep72 is essential for the localization of CG-NAP and Kizuna. Cep72 is also involved in γTuRC recruitment to the centrosome and CG-NAP confers the microtubule-nucleation activity on the γTuRCs. During mitosis, Cep72-mediated microtubule organization is important for converging spindle microtubules to the centrosomes, which is needed for chromosome alignment and tension generation between kinetochores. Our findings show that Cep72 is the key protein essential for maintaining microtubule-organizing activity and structural integrity of the centrosome.

Original languageEnglish (US)
Pages (from-to)2066-2076
Number of pages11
JournalEMBO Journal
Issue number14
StatePublished - Jul 22 2009
Externally publishedYes


  • Centrosome
  • Cep72
  • Kizuna
  • Microtubule
  • Mitotic spindle

ASJC Scopus subject areas

  • General Neuroscience
  • Molecular Biology
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology


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