TY - JOUR
T1 - Characterization of insulin-like growth factor binding proteins of cultured rat astroglial and neuronal cells
AU - Ocrant, Ian
AU - Pham, Hung
AU - Oh, Youngman
AU - Rosenfeld, Ron G.
N1 - Funding Information:
This work was supported in part by NIH grants DK-28229, DK-36054, and CA-42106 (RGR). RGR is the recipient of a Research Career Development Award from the NIH (DK-01275). IO is the recipient of a National Research Service Award from the NIH (DK-08075). We greatly appreciate the assistance of Dr. L.F. Eng and Yuen-Ling Lee, of the Veteran's Administration Medical Center (Palo Alto, CA) in preparing the rat astrocyte cultures and astrocyte conditioned medium.
PY - 1989/3/31
Y1 - 1989/3/31
N2 - Insulin-like growth factor binding proteins produced by cultured rat neurons, astrocytes, and rat cell lines BRL-3A and B104 were compared to binding proteins found in rat serum, using affinity labeling, deglycosylation, and Western ligand blotting studies. Each source elaborated an unique pattern of heterogeneous binding proteins. Some of the binding proteins from different sources behaved similarly in each experimental system suggesting that subsets of these binding proteins may be structurally related. In particular, our data suggest that cultured astrocytes and neurons make the major binding protein produced by BRL-3A cells.
AB - Insulin-like growth factor binding proteins produced by cultured rat neurons, astrocytes, and rat cell lines BRL-3A and B104 were compared to binding proteins found in rat serum, using affinity labeling, deglycosylation, and Western ligand blotting studies. Each source elaborated an unique pattern of heterogeneous binding proteins. Some of the binding proteins from different sources behaved similarly in each experimental system suggesting that subsets of these binding proteins may be structurally related. In particular, our data suggest that cultured astrocytes and neurons make the major binding protein produced by BRL-3A cells.
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U2 - 10.1016/0006-291X(89)92254-7
DO - 10.1016/0006-291X(89)92254-7
M3 - Article
C2 - 2467673
AN - SCOPUS:0024521956
SN - 0006-291X
VL - 159
SP - 1316
EP - 1322
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -