@article{1076c94c3abe496e9f09333e8f786de6,
title = "Characterization of placental and decidual cell development in early pregnancy loss by single-cell RNA sequencing",
abstract = "Background: Early pregnancy loss (EPL) presents as sporadic or recurrent miscarriage during the first trimester. In addition to chromosomal defects, EPL may result from impairment of the placental-decidual interface at early gestational age due to gene-environmental interactions. Methods: To better understand the pathogenesis associated with this impairment, cell development in chorionic villi and decidua of different forms of EPL (sporadic or recurrent) was investigated with single-cell RNA sequencing and compared to that of normal first-trimester tissue. Results: Unique gene expression signatures were obtained for the different forms of EPL and for normal tissue and the composition of placental and decidual cell clusters in each form was established. In particular, the involvement of macrophages in the EPL phenotypes was identified revealing an immunoactive state. Conclusion: Differential gene expression and unique marker genes among cell clusters from chorionic villi and decidua of miscarried and normal pregnancies, may lead to identification of biomarker for EPL.",
keywords = "First trimester, Miscarriage, Pregnancy, Single-cell RNA sequencing, Transcriptomics",
author = "Yuhua Zheng and Jing Pan and Chenglai Xia and Haiying Chen and Huadong Zhou and Weina Ju and Jerzy Wegiel and Leslie Myatt and Roberts, {James M.} and Xiaoling Guo and Nanbert Zhong",
note = "Funding Information: This project was supported in part by the Foshan Medicine Dengfeng Project (2019–2021), the New York State Research Foundation for Mental Hygiene (914-3280, 914-3257), and the National Institutes of Health (NIH/NICHD 1R01HD094381). We would like to thank faculty members of the Foshan Maternity and Child Healthcare Hospital and the New York State Institute for Basic Research in Developmental Disabilities who have supported this project. We would also like to thank Guangzhou Huaying Health ( www.huayinlab.com ) for technical support and Ms. Maureen Marlow for editorial assistance in preparation of the manuscript. We would like to thank faculty members of the Foshan Maternity 95 and Child Healthcare Hospital and the New York State Institute for Basic Research in Developmental Disabilities who have supported this project. We would also like to thank Guangzhou Huaying Health ( www.huayinlab.com ) for technical support and Ms. Maureen Marlow for editorial assistance in preparation of the manuscript. Funding Information: This project was supported in part by the Foshan Medicine Dengfeng Project (2019–2021), the New York State Research Foundation for Mental Hygiene (914-3280, 914-3257), and the National Institutes of Health (NIH/NICHD 1R01HD094381). We would like to thank faculty members of the Foshan Maternity and Child Healthcare Hospital and the New York State Institute for Basic Research in Developmental Disabilities who have supported this project. We would also like to thank Guangzhou Huaying Health (www.huayinlab.com) for technical support and Ms. Maureen Marlow for editorial assistance in preparation of the manuscript. We would like to thank faculty members of the Foshan Maternity 95 and Child Healthcare Hospital and the New York State Institute for Basic Research in Developmental Disabilities who have supported this project. We would also like to thank Guangzhou Huaying Health (www.huayinlab.com) for technical support and Ms. Maureen Marlow for editorial assistance in preparation of the manuscript. Funding Information: This project was supported in part by the Foshan Medicine Dengfeng Project (2019–2021), the New York State Research Foundation for Mental Hygiene (914-3280, 914-3257), and the National Institutes of Health (NIH/NICHD 1R01HD094381). Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = dec,
doi = "10.1186/s13578-022-00904-5",
language = "English (US)",
volume = "12",
journal = "Cell and Bioscience",
issn = "2045-3701",
publisher = "Society of Chinese Bioscientists in America",
number = "1",
}