Abstract
The innate cellular response to virus particle entry in non-immune cells requires the transcriptional activity of interferon regulatory factor 3 (IRF-3), but not production of type I interferon (IFN). Here, we characterize the IFN-independent innate cellular response to virus-derived stimuli in Vero cells, a monkey kidney epithelial cell line deficient for IFN production. We provide evidence that Vero cells are deficient in their ability to mount an IRF-3-dependent, IFN-independent antiviral response against either incoming virus particles or polyinosinic:polycytidylic acid (pIC), a dsRNA mimetic. We further demonstrate that abundance of IRF-3 protein is a determinant in the pIC-mediated antiviral signalling pathway. These observations further characterize the permissive nature of Vero cells to viral infection, and highlight the crucial involvement of IRF-3 in the innate antiviral response.
Original language | English (US) |
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Pages (from-to) | 393-399 |
Number of pages | 7 |
Journal | Molecular Immunology |
Volume | 46 |
Issue number | 3 |
DOIs | |
State | Published - Jan 2009 |
Externally published | Yes |
Keywords
- IFN
- IRF-3
- Innate immunity
- Vero cells
- Virus
ASJC Scopus subject areas
- Immunology
- Molecular Biology