Chemically phosphorylated protamine: A new substrate for the study of phosphoprotein phosphatase activity

Buddy Ullman; Robert L. Perlman

doi:10.1016/0006-291X(75)90705-6

Chemically phosphorylated protamine: A new substrate for the study of phosphoprotein phosphatase activity

Buddy Ullman, Robert L. Perlman

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

A method for the chemical phosphorylation of seryl residues in protamine has been developed. Both non-radioactive and [³³P]phosphoprotamine have been prepared and characterized. Phosphoprotein phosphatases from a variety of mammalian tissues release inorganic phosphate from phosphoprotamine. Chemically phosphorylated protamine is a useful new substrate for the study of these phosphoprotein phosphatases.

Original language	English (US)
Pages (from-to)	424-431
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	63
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(75)90705-6
State	Published - Mar 17 1975
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(75)90705-6

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abstract = "A method for the chemical phosphorylation of seryl residues in protamine has been developed. Both non-radioactive and [33P]phosphoprotamine have been prepared and characterized. Phosphoprotein phosphatases from a variety of mammalian tissues release inorganic phosphate from phosphoprotamine. Chemically phosphorylated protamine is a useful new substrate for the study of these phosphoprotein phosphatases.",

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AB - A method for the chemical phosphorylation of seryl residues in protamine has been developed. Both non-radioactive and [33P]phosphoprotamine have been prepared and characterized. Phosphoprotein phosphatases from a variety of mammalian tissues release inorganic phosphate from phosphoprotamine. Chemically phosphorylated protamine is a useful new substrate for the study of these phosphoprotein phosphatases.

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Chemically phosphorylated protamine: A new substrate for the study of phosphoprotein phosphatase activity

Abstract

ASJC Scopus subject areas

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