Two novel catechol 1,2-dioxygenase (C 1,2-O) genes have been isolated from an Acinetobacter radioresistens strain that grows on phenol or benzoate as sole carbon and energy source. Designated as catAA and catAB, they encode proteins composed of 314 and 306 amino acids, whose deduced sequences indicate that they have approximately 53% identity, whereas their NH2-terminal and COOH-terminal regions have no sequences in common. This may explain their different thermal and pH stability. Polyclonal antibodies raised against an amino-terminal CatAA peptide or the whole CatAB protein were used to establish their inducible and differential expression patterns upon bacterial growth in phenol or benzoate. The CatAA protein (IsoA) was induced by both phenol and benzoate though with different kinetics, whereas the catAB product (IsoB) was constitutively produced at low levels that increased only during growth in the presence of benzoate.
- Acinetobacter radioresistens S13
- Catechol 1,2-dioxygenase
- Expression patterns
ASJC Scopus subject areas
- Molecular Biology