TY - JOUR
T1 - Cloning, expression, and chromosomal localization of human long-chain fatty acid-CoA ligase 4 (FACL4)
AU - Cao, Yang
AU - Traer, Elie
AU - Zimmerman, Guy A.
AU - McIntyre, Thomas M.
AU - Prescott, Stephen M.
N1 - Funding Information:
We are grateful to Rebecca R. Shepard and Aurelia Meloni-Ehrig at the FISH core facility and Margaret Robertson at the nucleotide sequencing core facility of the University of Utah for their excellent technical assistance. We also thank Diana Lim for her help in preparing the manuscript. This study was supported by the Huntsman Cancer Institute and by Grant CA42014 from the National Cancer Institute. It was also supported by the Department of Defense (DAMD17-94-J-4259) and a postdoctoral fellowship HL09775 from the National Institutes of Health (Y. Cao).
PY - 1998/4/15
Y1 - 1998/4/15
N2 - Long-chain fatty acid-CoA ligase (also called fatty acid acyl-CoA synthetase) plays an essential role in lipid biosynthesis and fatty acid degradation. We report herein the cDNA cloning of the human long-chain fatty acid-CoA ligase 4 from a brain library. The cDNA encodes a functional long- chain fatty acid-CoA ligase that shows preference for arachidonic acid as substrate. We also studied the tissue distribution of gene expression by Northern hybridization. Human placenta, brain, testis, ovary, spleen, and adrenal cortex have the highest levels of expression of the long-chain fatty acid-CoA ligase 4, whereas the GI system has the lowest. Finally, this gene was localized to chromosome Xq23 in human by FISH analysis.
AB - Long-chain fatty acid-CoA ligase (also called fatty acid acyl-CoA synthetase) plays an essential role in lipid biosynthesis and fatty acid degradation. We report herein the cDNA cloning of the human long-chain fatty acid-CoA ligase 4 from a brain library. The cDNA encodes a functional long- chain fatty acid-CoA ligase that shows preference for arachidonic acid as substrate. We also studied the tissue distribution of gene expression by Northern hybridization. Human placenta, brain, testis, ovary, spleen, and adrenal cortex have the highest levels of expression of the long-chain fatty acid-CoA ligase 4, whereas the GI system has the lowest. Finally, this gene was localized to chromosome Xq23 in human by FISH analysis.
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U2 - 10.1006/geno.1998.5268
DO - 10.1006/geno.1998.5268
M3 - Article
C2 - 9598324
AN - SCOPUS:0032522157
SN - 0888-7543
VL - 49
SP - 327
EP - 330
JO - Genomics
JF - Genomics
IS - 2
ER -