TY - JOUR
T1 - Comparison of metabolic profiles and bioactivities of the leaves of three edible Congolese Hibiscus species
AU - Kapepula, Paulin Mutwale
AU - Kabamba Ngombe, Nadege
AU - Tshisekedi Tshibangu, Pascal
AU - Tsumbu, César
AU - Franck, Thierry
AU - Mouithys-Mickalad, Ange
AU - Mumba, Dieudonné
AU - Tshala-Katumbay, Désiré
AU - Serteyn, Didier
AU - Tits, Monique
AU - Angenot, Luc
AU - Kalenda, Pascal Dibungi T.
AU - Frédérich, Michel
N1 - Publisher Copyright:
© 2017 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2017/12/17
Y1 - 2017/12/17
N2 - Methanolic and dichloromethane extracts from the leaves of Congolese Hibiscus species were characterised by chromatographic and spectroscopic techniques and their in vitro biochemical activities against ROS production were evaluated in cellular models and on an enzyme, myeloperoxidase (MPO), involved in inflammation. Hibiscus acetosella has a chemical fingerprint different from Hibiscus cannabinus and Hibiscus sabdariffa both having similar fingerprints. Major compounds were polyphenols, represented mainly by caffeoyl-hydroxycitric acid for H. acetosella and neochlorogenic acid for the two other species. All extracts displayed high cellular antioxidant activity with IC50 values ranging from 0.5 to 3 μg mL−1 using lucigenin on neutrophils. Dichloromethane extracts showed more efficient effects on extracellular ROS production and MPO activity. Antioxidant and anti-inflammatory activities of caffeoyl-hydroxycitric acid were significantly higher than those of neochlorogenic acid. The bioactivities of Hibiscus species were positively correlated with their phytochemical content and could justify their use as local nutraceutical resources and medicines.
AB - Methanolic and dichloromethane extracts from the leaves of Congolese Hibiscus species were characterised by chromatographic and spectroscopic techniques and their in vitro biochemical activities against ROS production were evaluated in cellular models and on an enzyme, myeloperoxidase (MPO), involved in inflammation. Hibiscus acetosella has a chemical fingerprint different from Hibiscus cannabinus and Hibiscus sabdariffa both having similar fingerprints. Major compounds were polyphenols, represented mainly by caffeoyl-hydroxycitric acid for H. acetosella and neochlorogenic acid for the two other species. All extracts displayed high cellular antioxidant activity with IC50 values ranging from 0.5 to 3 μg mL−1 using lucigenin on neutrophils. Dichloromethane extracts showed more efficient effects on extracellular ROS production and MPO activity. Antioxidant and anti-inflammatory activities of caffeoyl-hydroxycitric acid were significantly higher than those of neochlorogenic acid. The bioactivities of Hibiscus species were positively correlated with their phytochemical content and could justify their use as local nutraceutical resources and medicines.
KW - Caffeoyl-hydroxycitric acid
KW - Hibiscus acetosella
KW - Hibiscus cannabinus
KW - Hibiscus sabdariffa
KW - myeloperoxidase
KW - neochlorogenic acid
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U2 - 10.1080/14786419.2017.1305382
DO - 10.1080/14786419.2017.1305382
M3 - Article
C2 - 28322589
AN - SCOPUS:85015785902
SN - 1478-6419
VL - 31
SP - 2885
EP - 2892
JO - Natural Product Research
JF - Natural Product Research
IS - 24
ER -