Corticosterone dysregulation exacerbates disease progression in the R6/2 transgenic mouse model of Huntington's disease

Brett D. Dufour, Jodi L. McBride

    Research output: Contribution to journalArticlepeer-review

    14 Scopus citations


    Huntington's disease (HD) is a genetic neurological disorder that causes severe and progressive motor, cognitive, psychiatric, and metabolic symptoms. There is a robust, significant elevation in circulating levels of the stress hormone, cortisol, in HD patients; however, the causes and consequences of this elevation are largely uncharacterized. Here, we evaluated whether elevated levels of corticosterone, the rodent homolog of cortisol, contributed to the development of symptomology in transgenic HD mice. Wild-type (WT) and transgenic R6/2 mice were given either 1) adrenalectomy with WT-level corticosterone replacement (10 ng/ml), 2) adrenalectomy with high HD-level corticosterone replacement (60 ng/ml), or 3) sham surgery without replacement. R6/2 mice on HD-level replacement showed severe and rapid weight loss (p < 0.05) and a shorter latency to death (p < 0.01) relative to the HD mice on WT-level replacement. We further evaluated basal and stress-induced levels of circulating corticosterone in R6/2 mice throughout the course of their life. We found that R6/2 transgenic HD mice display a spontaneous elevation in circulating corticosterone levels that became significant at 10 weeks of age. Furthermore, we identified significant dysregulation of circadian rhythmicity of corticosterone release measured over a 24 h period compared to wild-type controls. Unexpectedly, we found that R6/2 transgenic mice show a blunted corticosterone response to restraint stress, compared to wild-type mice. Together, these data provide further evidence that HPA-axis activity is abnormal in R6/2 mice, and highlight the important role that cortisol plays in HD symptom development. Our findings suggest that cortisol-reducing therapeutics may be of value in improving HD patient quality of life.

    Original languageEnglish (US)
    Pages (from-to)308-317
    Number of pages10
    JournalExperimental Neurology
    StatePublished - Sep 1 2016


    • Circadian dysfunction
    • Corticosterone
    • Cortisol
    • HPA-axis
    • Huntington's disease
    • R6/2 transgenic mice

    ASJC Scopus subject areas

    • Neurology
    • Developmental Neuroscience


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