Abstract
Cerebrospinal fluid (CSF) matrix biomarkers have become increasingly valuable surrogate markers of neuropsychiatric diseases in research and clinical practice. In contrast, CSF cells have been rarely investigated due to their relative scarcity and fragility, and lack of common collection and cryopreservation protocols, with limited exceptions for neurooncology and primary immune-based diseases like multiple sclerosis. the advent of a microfluidics-based multi-omics approach to studying individual cells has allowed for the study of cellular phenotyping, intracellular dynamics, and intercellular relationships that provide multidimensionality unable to be obtained through acellular fluid-phase analyses. challenges to cell-based research include site-to-site differences in handling, storage, and thawing methods, which can lead to inaccuracy and inter-assay variability.
Original language | English (US) |
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Article number | 71 |
Journal | Journal of Neuroinflammation |
Volume | 21 |
Issue number | 1 |
DOIs | |
State | Published - Dec 2024 |
Keywords
- 10x
- Alzheimer’s disease
- Cerebrospinal fluid
- Methods
- Neuroimmunology
- Omics
- Single-cell
- Transcriptomics
ASJC Scopus subject areas
- General Neuroscience
- Immunology
- Neurology
- Cellular and Molecular Neuroscience