TY - JOUR
T1 - Damage to Isolated Hearts by Oxygenators
AU - Brown, A. Hedley
AU - Niles, Nelson R.
AU - Braimbridge, Mark V.
AU - Austen, W. Gerald
N1 - Funding Information:
From the Department of Surgery, Harvard Medical School, and the General Surgical Services, Massachusetts General Hospital, Boston, Mass. Supported in part by U.S. Public Health Service Grant No. HE-06664 (HEPP). Accepted for publication Dec. 22. 1971. Address reprint requests to Dr. Austen, Department of Surgery, Massachusetts General Hospital. Boston, Mass. 02114.
PY - 1972
Y1 - 1972
N2 - The developed tension, compliance, and contractile velocity of isolated isovolumetric canine hearts, with myocardial biopsies examined by succinic dehydrogenase, acid hematein, and birefringence techniques, have been employed to investigate currently used perfusion methods. Ten isolated hearts perfused from a standard oxygenator, 11 from a mini-oxygenator, 12 from an oxygenator perfusing the whole body as well as the heart, and 12 cross-perfused from another dog were thus compared. Performance tests showed cross-perfusion to be the least damaging, followed by whole-body perfusion plus oxygenator, perfusion with the large oxygenator, and perfusion with the mini-oxygenator, in that order. Histochemistry showed deterioration of the oxygenator-supported hearts, but there was no deterioration in those supported by cross-perfusion. The cross-perfused isolated isovolumetric heart should thus be a good model for prolonged tests involving ventricular function. Drill biopsy appears to be a reasonable method for detecting early myocardial damage.
AB - The developed tension, compliance, and contractile velocity of isolated isovolumetric canine hearts, with myocardial biopsies examined by succinic dehydrogenase, acid hematein, and birefringence techniques, have been employed to investigate currently used perfusion methods. Ten isolated hearts perfused from a standard oxygenator, 11 from a mini-oxygenator, 12 from an oxygenator perfusing the whole body as well as the heart, and 12 cross-perfused from another dog were thus compared. Performance tests showed cross-perfusion to be the least damaging, followed by whole-body perfusion plus oxygenator, perfusion with the large oxygenator, and perfusion with the mini-oxygenator, in that order. Histochemistry showed deterioration of the oxygenator-supported hearts, but there was no deterioration in those supported by cross-perfusion. The cross-perfused isolated isovolumetric heart should thus be a good model for prolonged tests involving ventricular function. Drill biopsy appears to be a reasonable method for detecting early myocardial damage.
UR - http://www.scopus.com/inward/record.url?scp=0015358097&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0015358097&partnerID=8YFLogxK
U2 - 10.1016/S0003-4975(10)65175-2
DO - 10.1016/S0003-4975(10)65175-2
M3 - Article
C2 - 5037830
AN - SCOPUS:0015358097
SN - 0003-4975
VL - 13
SP - 575
EP - 588
JO - Annals of Thoracic Surgery
JF - Annals of Thoracic Surgery
IS - 6
ER -