TY - JOUR
T1 - Decrease in salivary secretion by radiation mediated by nitric oxide and prostaglandins
AU - De La Cal, Carolina
AU - Lomniczi, Alejandro
AU - Mohn, Claudia E.
AU - De Laurentiis, Andrea
AU - Casal, Mariana
AU - Chiarenza, Ana
AU - Paz, Dante
AU - McCann, Samuel M.
AU - Rettori, Valeria
AU - Elverdín, Juan Carlos
PY - 2006/8
Y1 - 2006/8
N2 - Objective: In the present work, we evaluated the effect of exposing the submandibular glands (SMG) to radiation, studying different functional parameters such as salivary secretion, nitric oxide (NO) production, reactive oxygen species formation, prostaglandin (PGE) content and apoptosis. Methods: We irradiated rats in the head and neck region with a single dose of γ-ray radiation of 15 Gy. Two hours after radiation, we measured norepinephrine- induced salivary secretion. After that, the SMG were dissected, and in this tissue, we measured the activity of NO synthase (NOS), the PGE content, the amount of reactive oxygen species, apoptotic cells and mitochondrial inducible NOS (iNOS) expression. Results: We found that radiation decreased salivary secretion when 10 and 30 μg/kg of norepinephrine was administered via the right femoral vein. We observed that iNOS activity was reduced and PGE content increased after radiation in SMG, indicating that NO and PGEs may participate in salivary secretion. The expression of mitochondrial NOS was increased after radiation leading to the formation of large amounts of NO that acts as a proapoptotic signal. In fact, we observed an augmentation in apoptotic cells. In this study, we also observed an increase in lipid peroxidation induced by radiation that may contribute to tissue damage. Conclusions: Our results indicate that radiation induced a decrease in salivary secretion and SMG iNOS activity, meanwhile the PGE content, the lipid peroxidation and apoptosis increased in the tissue. These modifications decrease salivary secretion.
AB - Objective: In the present work, we evaluated the effect of exposing the submandibular glands (SMG) to radiation, studying different functional parameters such as salivary secretion, nitric oxide (NO) production, reactive oxygen species formation, prostaglandin (PGE) content and apoptosis. Methods: We irradiated rats in the head and neck region with a single dose of γ-ray radiation of 15 Gy. Two hours after radiation, we measured norepinephrine- induced salivary secretion. After that, the SMG were dissected, and in this tissue, we measured the activity of NO synthase (NOS), the PGE content, the amount of reactive oxygen species, apoptotic cells and mitochondrial inducible NOS (iNOS) expression. Results: We found that radiation decreased salivary secretion when 10 and 30 μg/kg of norepinephrine was administered via the right femoral vein. We observed that iNOS activity was reduced and PGE content increased after radiation in SMG, indicating that NO and PGEs may participate in salivary secretion. The expression of mitochondrial NOS was increased after radiation leading to the formation of large amounts of NO that acts as a proapoptotic signal. In fact, we observed an augmentation in apoptotic cells. In this study, we also observed an increase in lipid peroxidation induced by radiation that may contribute to tissue damage. Conclusions: Our results indicate that radiation induced a decrease in salivary secretion and SMG iNOS activity, meanwhile the PGE content, the lipid peroxidation and apoptosis increased in the tissue. These modifications decrease salivary secretion.
KW - Apoptosis
KW - Nitric oxide synthase
KW - Submandibular glands
KW - Xerostomia
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U2 - 10.1159/000093194
DO - 10.1159/000093194
M3 - Article
C2 - 16691037
AN - SCOPUS:33746849277
SN - 1021-7401
VL - 13
SP - 19
EP - 27
JO - NeuroImmunoModulation
JF - NeuroImmunoModulation
IS - 1
ER -