Demineralization of resected root-ends with methylene blue dye

Leslie A. Morgan, J. Craig Baumgartner

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Objectives. This study evaluated the ability of methylene blue solutions of different concentration, pH, and time exposure to remove the smear layer from resected root-ends. Study design. Resected root-ends were treated with either 2% methylene blue dye for intervals of 5 to 11 minutes or 1% methylene blue for 7 to 9 minutes and examined with a scanning electron microscope for smear layer removal. Two minute applications of saline solution and 50% citric acid served as controls. Results. All methylene blue solutions were found to be ineffective in removing the smear layer at all time intervals tested. An experimental solution of 1% methylene blue in 50% citric acid was found to predictably remove the smear layer during a 2 minute application and retained all the favorable staining characteristics of the dye. Fifty percent citric acid solutions applied for 3 minutes appeared to overdemineralize the root-end and gave poorer results than 2 minute applications. Conclusions. Smear layer removal was pH dependent. Methylene blue solutions are clinically unsuitable for smear layer removal.

Original languageEnglish (US)
Pages (from-to)74-78
Number of pages5
JournalOral surgery, oral medicine, oral pathology, oral radiology, and endodontics
Issue number1
StatePublished - 1997

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology
  • General Dentistry


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