Abstract
A real-time RT-PCR assay was designed to detect and differentiate norovirus genogroups I (GI) and II (GII), with primers and probes targeting the nonstructural polyprotein gene. Stool samples (n = 100) submitted for routine testing by the BioFire FilmArray® GI panel were also tested by the norovirus GI/GII real-time PCR assays. When compared to the FilmArray GI panel, the norovirus real-time PCR assay demonstrated a sensitivity of 77.5% (62/80) and specificity of 95% (19/20). Specimens yielding discordant results (n = 19) were tested at two outside laboratories for adjudication. Following discordant resolution, the adjusted sensitivity and specificity of the norovirus real-time PCR assays were 96.9% (63/65) and 100% (35/35), respectively. These results suggest that the real-time PCR assays are able to accurately detect and differentiate norovirus GI/GII from clinical stool specimens. Furthermore, our report highlights a potential issue with the specificity of the BioFire FilmArray® norovirus assay, which warrants additional investigation.
Original language | English (US) |
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Pages (from-to) | 325-327 |
Number of pages | 3 |
Journal | Diagnostic Microbiology and Infectious Disease |
Volume | 87 |
Issue number | 4 |
DOIs | |
State | Published - Apr 1 2017 |
Externally published | Yes |
Keywords
- Norovirus
- PCR
ASJC Scopus subject areas
- Microbiology (medical)
- Infectious Diseases